Journal ArticleDOI
Immunologic diagnosis of alpha-thalassemia traits.
TLDR
In this article, an antibody against Hb Bart's was produced in rabbits by repeated injections with Bart's from hydropic fetuses, without cross-reaction with Hb s A, A2, E, F and H. The test was positive in 21 % of the general subjects, corresponding to the prevalence of α-thalassemia.Abstract:
Frequently it is impossible to diagnose α-thalassemia-2 trait from hematologic findings, since the red cell morphology and the MCV are normal. In such cases, after the neonatal period Hb Bart's is not detectable by conventional electrophoreses and chromatography. Antibody against Hb Bart's was produced in rabbits by repeated injections with Hb Bart's- from Hb Bart's hydropic fetuses. The antibody was specific for Hb Bart's without cross-reaction with Hb s A, A2, E, F and H. By the capillary tube precipitin test Hb Bart's was demonstrable in 82% of 87 obligatory cases for α-thalassemia traits, 86 % in α-thalassemia-1 trait and 79% in α-thalassemia-2 trait. The test was positive in 21 % of the general subjects, corresponding to the prevalence of α-thalassemia in Bangkok. Thus the immunologic demonstration of Hb Bart's appears to offer a diagnostic screening test for α-thalassemia traits in the postneonatal period.read more
Citations
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Journal ArticleDOI
Detection of common deletional α-thalassemia-2 determinants by PCR
E. Baysal,Titus H.J. Huisman +1 more
TL;DR: A rapid approach to detect the two most common α‐thalassemia‐2 determinants by the polymerase chain reaction (PCR) technique, which takes a few hours to complete, is believed to provide a cost‐effective way to screen large numbers of blood samples in a relatively short time and can be used to identifyα‐thal‐2 heterozygote and homozygotes and compound heterozygotes in populations where such α‐gene defects are shown to exist at high frequencies.
Journal ArticleDOI
A PCR-based strategy to detect the common severe determinants of alpha thalassaemia.
TL;DR: This strategy should be useful in the development of screening programmes to identify carriers of α thalassaemia and prenatal diagnosis of the Hb Bart's hydrops fetalis syndrome for those populations in which this represents a major cause of perinatal death.
Journal ArticleDOI
5 α-Thalassaemia
TL;DR: The data summarized here provide the basis for accurately predicting the genotype in α-thalassaemia cases and thus enabling appropriate prenatal testing and a strategy for understanding the co-ordinate regulation of eukaryotic gene expression.
Journal ArticleDOI
Simple method for screening of α-thalassaemia 1 carriers
Chatchai Tayapiwatana,Surakit Kuntaruk,Thanusak Tatu,Sawitree Chiampanichayakul,Thongperm Munkongdee,Pranee Winichagoon,Suthat Fuchareon,Watchara Kasinrerk +7 more
TL;DR: Results indicate that this novel immunodiagnostic kit, in combination with red blood cell indices, is suitable for screening and ruling out mass populations for the presence of α-thalassaemia 1.
References
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Journal ArticleDOI
Differentiation of iron deficiency from thalassaemia trait by routine blood-count.
J.M. England,PatriciaM. Fraser +1 more
TL;DR: In 72 patients presenting with microcytosis, a simple discriminant function derived from the mean cell volume and the haemoglobin concentration correctly identified all but one of the cases studied (99%).
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Identification of a nondeletion defect in α-thalassemia.
TL;DR: A Chinese family in which analysis of the mode of inheritance of α-thalassemia-1 and hemoglobin-H disease suggests a lesion that is not due to structural-gene deletion, and molecular hybridization studies with synthetic radioactive DNA's complementary to α-globin mRNA sequences show that in addition to the usual deletion defect, a nondeletion defect produces the phenotype of α
Journal ArticleDOI
Deletion of α-globin genes in haemoglobin-H disease demonstrates multiple α-globin structural loci
Yuet Wai Kan,Andrée M. Dozy,Harold E. Varmus,J. M. Taylor,J. P. Holland,Luan Eng Lie-Injo,J. Ganesan,David Todd +7 more
TL;DR: The results firmly support the hypothesis that the number of α-globin structural genes in haemoglobin-H disease is duplicated and that α thalassaemia-1 and αThalassAemia-2 are caused by a defect of greater and lesser severity affecting alleles of a single α- globin locus.
Journal ArticleDOI
Haemoglobin h disease in thailand: a genetical study.
TL;DR: This disease became known here as inclusion-body anæmia, but was later identified as thalassæmia-Hgb H disease, first reported by Rigas et al.
Journal ArticleDOI
Haemoglobin Bart's in Saudi Arabia.
TL;DR: Findings indicate that elevated levels of Hb Bart's in this population are due to the presence of a thalassaemia, and points to an a‐thalassacmia genotype that is intermediate between the heterozygous state for α‐thalASSacmia 1 and Hb‐H disease.