Journal ArticleDOI
Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse
G.J. Wiepjes,F.J.A. Prop +1 more
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This article is published in Experimental Cell Research.The article was published on 1970-08-01. It has received 75 citations till now.read more
Citations
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Journal ArticleDOI
Maintenance and induction of morphological differentiation in dissociated mammary epithelium on floating collagen membranes
TL;DR: Dissociated normal mammary epithelial cells from prelactating mice were plated on different substrates in various medium-serum-hormone combinations to find conditions that would permit maintenance of morphological differentiation.
Book ChapterDOI
The Differentiated State of Normal and Malignant Cells or How to Define a “Normal” Cell in Culture
TL;DR: This chapter discusses the differentiated state of normal and malignant cells, and the extent and the composition of the extracellular matrix is an important differentiated trait of tendon cells.
Journal Article
The effect of estrone-progesterone treatment on cell proliferation kinetics of hormone-dependent GR mouse mammary tumors.
P Janik,P Briand,N R Hartmann +2 more
TL;DR: The results might indicate that estrone and progesterone treatment stimulated growth of hormone-dependent GR mouse mammary tumors mainly by an increase of growth fraction and cell production rate.
Journal ArticleDOI
To disaggregate or not to disaggregate injury and cell disaggregation, transient or permanent?
TL;DR: Methods for disaggregating tissues to obtain cells for primary cultures are reviewed and evaluated and include dissociation with crude and purified enzymes, depletion of cations, and manipulation of pH and osmolality.
Book ChapterDOI
Responses of mammary cells to hormones.
TL;DR: This chapter discusses the response of mammary cells to hormones, which reveals that the mammary epithelium in a culture medium responds to combinations of hormones similar to those needed in vivo.
References
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Journal ArticleDOI
Rotation-mediated histogenetic aggregation of dissociated cells. A quantifiable approach to cell interactions in vitro.
TL;DR: A cell aggregation procedure based on readily standardizable manipulations is described, whereby cells dissociated enzymatically from embryonic tissues may be aggregated in various combinations and concentrations into developmentally effective multicellular structures; it is suitable for analyzing mutual reactions of cells and their responses to diverse environmental conditions.
Book ChapterDOI
The Isolation of Living Cells from Animal Tissues
TL;DR: Cell cultures provide a cleaner system than the conventional methods of explant cultures, where the core of the explant, much denser than the outgrowth, may degenerate to some extent and may become an unaccountable source of nutrients or catabolic products.
Journal ArticleDOI
An improved method for the isolation and quantitative cultivation of embryonic cells.
TL;DR: A method is described for dissociating the heart ventricles and skeletal muscle of 12- to 14-day-old chick embryos and for cultivating quantitatively the free cells thus obtained and some morphological and functional aspects relevant to the development of the culture methods are discussed.
Journal ArticleDOI
Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse.
Journal ArticleDOI
Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse. II. Observations on the secretory activity.
TL;DR: Cultures of mammary epithelium of the adult mouse can grow and organize when associated with plain connective tissue, and experimental evidence is given showing that milk-fat production is possibly under the specific control of the adipose tissue.