Journal ArticleDOI
Improved ultracentrifuge cells for high-speed sedimentation equilibrium studies with interference optics
TLDR
The increased optical reliability of the side-access cells helps to extend the routine application of meniscus depletion type molecular weight determinations to include molecules with sizes around 10,000 daltons.About:
This article is published in Analytical Biochemistry.The article was published on 1970-03-01. It has received 112 citations till now.read more
Citations
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Journal ArticleDOI
Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques.
TL;DR: Least-squares analysis of experimental data from the analytical ultracentrifuge is discussed in detail, with particular attention to the use of interference optics in studying nonideal self-associating macromolecular systems.
Journal ArticleDOI
Modern analytical ultracentrifugation in protein science: A tutorial review
TL;DR: Sedimentation equilibrium of proteins, including membrane proteins and glycoproteins, is the method of choice for molar mass determinations and the study of self‐association and heterogeneous interactions, such as protein–protein, protein–nucleic acid, and protein–small molecule binding.
Journal ArticleDOI
Mechanism of glutamate receptor desensitization
TL;DR: Using the GluR2 AMPA-sensitive glutamate receptor, it is shown that the ligand-binding cores form dimers and that stabilization of the intradimer interface by either mutations or allosteric modulators reduces desensitization.
Book ChapterDOI
Analytical Ultracentrifugation: Sedimentation Velocity and Sedimentation Equilibrium
TL;DR: The fluorescence system provides a new way to extend the scope of AUC to probe the behavior of biological molecules in complex mixtures and at high solute concentrations to provide a detailed picture of the nature of the species present in solution and their interactions.
Journal ArticleDOI
Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decomposition.
Jennifer Vistica,Julie Dam,Andrea Balbo,Emine Yikilmaz,Roy A. Mariuzza,Tracey A. Rouault,Peter Schuck +6 more
TL;DR: The present study proposes computational tools, implemented in the software SEDPHAT, for the global analysis of equilibrium data at multiple rotor speeds with multiple concentrations and multiple optical detection methods, and an unbiased scale for determining molar mass distributions of noninteracting species.
References
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Journal ArticleDOI
Rapid Attainment of Sedimentation Equilibrium
Journal ArticleDOI
Rapid Determination of Molecular Weights of Peptides and Proteins
TL;DR: It was felt worthwhile to repeat the experiments of Van Holde and Baldwin with shorter columns, that is, small distances between meniscus and base, to reduce the time required to attain equilibrium.
Journal ArticleDOI
Studies of self-associating systems by equilibrium ultracentrifugation.
TL;DR: A simple method of detecting heterogeneitywithin limits-in associating systems and therefore, by implication, of noting reversibility is reemphasized and methods of estimating stoichiometries in favorable cases and for evaluating association constants of nonideal systems are presented.