Journal ArticleDOI
In response--a modified staining protocol for Smoothelin immunostaining.
TLDR
A study assessing the utility of Smoothelin immunostaining in distinguishing muscularis mucosa (MM) from muscularis propria (MP) in 103 urinary bladder specimens, including 90 TURBTS and 13 cystectomies (results yet to be published).Abstract:
Dear Editor, In response to the article by Lindh et al. [1] in which they have discussed the utility of Smoothelin and have concluded that Smoothelin should be used cautiously, we would like to share our experience on the usefulness of Smoothelin staining. We performed a study assessing the utility of Smoothelin immunostaining in distinguishing muscularis mucosa (MM) from muscularis propria (MP) in 103 urinary bladder specimens, including 90 TURBTS and 13 cystectomies (results yet to be published). Immunohistochemical staining was performed using the Dako Autostainer Plus platform (Dako, Glostrup, Denmark) in order to validate the anti-Smoothelin primary antibody (clone R4A; Menarini Diagnostics, Wokingham, UK) for purposes of our study. Heat-induced epitope retrieval (HIER) was performed using the Dako PT link (Dako). Sections were incubated in the appropriate antigen retrieval buffer for 20 min at 97°C and were then allowed to cool to 65°C before transferring to distilled water. Two antigen retrieval buffers were used to establish the optimal HIER protocol. These HIER buffers were as follows:read more
Citations
More filters
Journal ArticleDOI
Challenges in Pathologic Staging of Bladder Cancer: Proposals for Fresh Approaches of Assessing Pathologic Stage in Light of Recent Studies and Observations Pertaining to Bladder Histoanatomic Variances
TL;DR: The muscularis propria (MP), besides being the essential histologic landmark for assigning pT2 stage category, is also considered a surrogate for the adequacy of TUR, furthering the importance of identifying its presence in TUR specimens.
Journal ArticleDOI
Contemporary Grading and Staging of Urothelial Neoplasms of the Urinary Bladder: New Concepts and Approaches to Challenging Scenarios.
TL;DR: In this paper, the authors provide an overview of the challenges in grading and staging of urothelial neoplasm, including the most crucial factors in risk stratification and management; both necessitate optimal accuracy and consistency.
Journal ArticleDOI
pT1 high-grade bladder cancer: histologic criteria, pitfalls in the assessment of invasion, and substaging
Maria Rosaria Raspollini,Rodolfo Montironi,Roberta Mazzucchelli,Alessia Cimadamore,Liang Cheng,Antonio Lopez-Beltran +5 more
TL;DR: The aim of this review paper is to provide the practicing pathologist with the state of the art of morphological and immunohistochemical features useful for the diagnosis of early invasive bladder carcinomas, including practical clues on how to avoid relevant interpretative pitfalls, and to summarize the current status of pT1 substaging.
Journal ArticleDOI
Diagnostic value of smoothelin and vimentin in differentiating muscularis propria from muscularis mucosa of bladder carcinoma.
TL;DR: Evaluation of diagnostic value of smoothelin and vimentin expression both singly and in combination for differentiation between MM and MP found moderate to strong smoothelins and negative vimentsin offered 100% sensitivity and 100% specificity towards the identification of MP.
Journal ArticleDOI
Proteomics as a Complementary Technique to Characterize Bladder Cancer.
TL;DR: In this article, the authors introduce epidemiology and the conventional classifications to review the molecular classification of bladder cancer, highlighting the contributions of proteomics, and discuss selected proteins for the comprehension of the pathophysiological mechanisms of BC.
References
More filters
Journal ArticleDOI
Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma.
TL;DR: Evaluating the expression of different markers in myofibroblasts and nonvascular smooth muscle cells in 15 cases of invasive bladder carcinoma suggests that immunohistochemistry, using a panel composed of desmin, smoothelin, and vimentin, may be potentially useful for staging of bladder cancer.
Journal ArticleDOI
Diagnostic utility of antibody to smoothelin in the distinction of muscularis propria from muscularis mucosae of the urinary bladder: a potential ancillary tool in the pathologic staging of invasive urothelial carcinoma.
Gladell P. Paner,Steven S. Shen,Shawn Lapetino,Girish Venkataraman,Güliz A. Barkan,Marcus L. Quek,Jae Y. Ro,Mahul B. Amin +7 more
TL;DR: The relatively distinct immunohistochemical staining pattern of smoothelin between MP and MM (including its hyperplastic forms) makes it a robust and attractive marker to be incorporated in the contemporary diagnostic armamentarium for the sometimes difficult area of staging bladder urothelial carcinoma.
Journal ArticleDOI
Pitfalls in the use of smoothelin to identify muscularis propria invasion by urothelial carcinoma.
TL;DR: The data confirm the relatively distinct staining pattern of smoothelin between MM and MP, however, caution should be maintained while using smoothelins immunohistochemistry as a diagnostic tool for MP invasion.
Journal ArticleDOI
Intrarenal ectopic adrenal tissue and renal-adrenal fusion: a report of nine cases.
TL;DR: Key morphological features of intrarenal ectopic adrenal tissue and renal–adrenal fusion along with histological pitfalls and its differential diagnoses are summarized.
Journal ArticleDOI
Detection of smoothelin expression in the urinary bladder is strongly dependent on pretreatment conditions: a critical analysis with possible consequences for cancer staging.
TL;DR: Smoothhelin IHC is strongly dependent on epitope retrieval, and smoothelin staining did not discriminate reliably between MP and MM with any of the tested pretreatment protocols.