Journal ArticleDOI
Increase of catechol-O-methyltransferase activity in rat brain microglia after intrastriatal infusion of fluorocitrate, a glial toxin
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TLDR
This is the first report indicating that microglia contains COMT activity which may be increased in pathological conditions, and the elevation of COMT immunoreactivity co-localized to microglial cells, but not to astrocytes.About:
This article is published in Neuroscience Letters.The article was published on 1997-07-25. It has received 25 citations till now. The article focuses on the topics: Catechol-O-methyl transferase & Neuroglia.read more
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Journal Article
Catechol-O-methyltransferase (COMT): biochemistry, molecular biology, pharmacology, and clinical efficacy of the new selective COMT inhibitors.
Pekka T. Männistö,Seppo Kaakkola +1 more
TL;DR: The enzyme responsible for the O- methylation, catechol- O -methyltransferase (COMT) was partly purified and characterized by the same group as EC, which first described the enzyme-catalyzed O-methylation of catechlamines and other catechols in the late 1950s.
Journal ArticleDOI
Methylmercury Induces Acute Oxidative Stress, Altering Nrf2 Protein Level in Primary Microglial Cells
Mingwei Ni,Xin Li,Zhaobao Yin,Haiyan Jiang,Marta Sidoryk-Wegrzynowicz,Dejan Milatovic,Jiyang Cai,Michael Aschner +7 more
TL;DR: This study has demonstrated that microglial cells are exquisitely sensitive to MeHg and respond rapidly to Mehg by upregulating the Nrf2-mediated antioxidant response.
Journal ArticleDOI
Distribution of catechol-O-methyltransferase (COMT) proteins and enzymatic activities in wild-type and soluble COMT deficient mice
TL;DR: In this paper, a specific COMT antibody was used in immunohistochemical and confocal co-localization studies to explore the distribution of COMT in general in normal mice and MB-COMT in particular, in an SCOMT deficient mouse line.
Journal ArticleDOI
An interaction between the catechol O-methyltransferase and serotonin transporter promoter region polymorphisms contributes to tridimensional personality questionnaire persistence scores in normal subjects.
Jonathan Benjamin,Yamima Osher,Pesach Lichtenberg,Rachel Bachner-Melman,Inga Gritsenko,Moshe Kotler,Robert H. Belmaker,Vadim Valsky,Michael Drendel,Richard P. Ebstein +9 more
TL;DR: TPQ RD2 scores are examined in 577 normal subjects inventoried for two common genetic polymorphisms, the catechol O-methyltransferase valine to methionine (val to met) amino acid substitution that determines high and low enzyme activity, and the serotonin transporter promoter region 44 bp deletion linked in some studies to harm avoidance or neuroticism.
Book ChapterDOI
The Catechol-O-Methyltransferase Gene: Its Regulation and Polymorphisms
TL;DR: The catechol-O-methyltransferase (COMT) gene is of significant interest to neuroscience, due to its role in modulating dopamine function as mentioned in this paper, and its expression is altered during normal brain development and in response to environmental stimuli.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
The Rat Brain in Stereotaxic Coordinates
George Paxinos,Charles Watson +1 more
TL;DR: This paper presents a meta-analyses of the determinants of earthquake-triggered landsliding in the Czech Republic over a period of 18 months in order to establish a probabilistic framework for estimating the intensity of the earthquake.
Book ChapterDOI
Isolation of subcellular organelles.
Brian Storrie,Edward A. Madden +1 more
TL;DR: This chapter explains the procedures for the isolation of nuclei, mitochondria, and lysosomes from cultured mammalian cell lines and compares enzyme activity in the presence and absence of detergent to assess organelle intactness.
Journal Article
Catechol-O-Methyl Transferase: Pharmacological Aspects and Physiological Role
Journal ArticleDOI
Injection of solutions into cerebral tissue: Relation between volume and diffusion
TL;DR: The relationship between the extent of diffusion of a solution and the volume injected has been investigated for cerebral tissue in anesthetized rats, and it was suggested that a volume no larger than 0.5 μl should be given if localization of the action of a drug is attempted.
Related Papers (5)
Catechol-O-methyltransferase (COMT): biochemistry, molecular biology, pharmacology, and clinical efficacy of the new selective COMT inhibitors.
Pekka T. Männistö,Seppo Kaakkola +1 more