Journal ArticleDOI
Increasing glucose determination range by flow injection analysis (FIA) using glucose oxidase immobilised on polyaniline
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TLDR
A Flow Injection Analysis for glucose using glucose oxidase (E.C.3.1.4) was developed and was able to measure glucose at levels from 5 to 500mM with a response time of 2min using 250μl of sample.Abstract:
A Flow Injection Analysis (FIA) for glucose using glucose oxidase (E.C.1.1.3.4) was developed. The enzyme was immobilised on polyaniline chemically synthetized on internal surface of silicon tube (2.0 mm of diameter and length from 1 to 5 meters), using glutaraldehyde as a bifunctional agent. The system was able to measure glucose at levels from 5 to 500mM with a response time of 2min using 250μl of sample. The system has been operated satisfactorily for one month with more than 300 assays with loss of 60% of activity.read more
Citations
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Conjugated polymer-based chemical sensors.
TL;DR: When considering new sensory technologies one should look to nature for guidance, as living organisms have developed the ultimate chemical sensors.
Journal ArticleDOI
Immobilization of horseradish peroxidase onto polyaniline polymers
TL;DR: Some chemically synthesized conductive polymers, based on polyaniline (PANI), were used as supports for Horseradish Peroxidase (HRP) immobilization and those with intermediate oxidation state (PANIG1, PANIG2 and PANIG3) were more suitable for HRP immobilization than that predominately reduced or oxidized.
Journal ArticleDOI
Thermal and morphological studies of chemically prepared emeraldine‐base‐form polyaniline powder
TL;DR: In this article, the thermal characteristics and chemical structures of polyaniline powder were studied by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).
Journal ArticleDOI
Immobilization of Aspergillus niger glucoamylase onto a polyaniline polymer
TL;DR: The immobilized glucoamylase (PANIG-GA) presented better performance than free enzyme when submitted to heat treatment, showing a 10 °C decrease on the optimal assay temperature.
Journal ArticleDOI
Properties of horseradish peroxidase immobilised onto polyaniline
TL;DR: Chemically synthesised polyaniline was used for horseradish peroxidase (HRP) immobilisation after activation with glutaraldehyde and in the best storage conditions the PANIG-HRP was 100% stable for 70 days.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Electrode systems for continuous monitoring in cardiovascular surgery.
Leland C. Clark,Champ Lyons +1 more
TL;DR: Improvement in the design and construction of electrode systems and their associated electronic instrumentation, together with the commercial development and availability of stable amplifiers and recorders, has now provided entirely satisfactory systems for the rapid and accurate measurement of blood pH, pCO2, and pOz.
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The enzyme electrode.
S. J. Updike,G. P. Hicks +1 more
TL;DR: The enzyme electrode is a miniature chemical transducer which functions by combining an electrochemical procedure with immobilized enzyme activity to measure the concentration of glucose in biological solutions and in the tissues in vitro.
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Miniaturized multi-enzyme biosensors integrated with pH sensors on flexible polymer carriers for in vivo applications
TL;DR: In this article, an electrochemical glucose sensor was integrated with a pH sensor on a flexible polyimide substrate for in vivo applications for short-term monitoring of glucose and pH in intensive care units and operating theatres, especially for neurosurgical applications.