Indigo formation by microorganisms expressing styrene monooxygenase activity.
TLDR
Characterization of an SMO- and styrene oxide isomerase (SOI)-negative transposon mutant of P. putida CA-3 and an SOI-negative N-methyl-N'-nitro-N-nitrosoguanidine Mutant reveals the involvement of both SMO and SOI in indole transformation to indigo.Abstract:
The transformation of indole to indigo by microorganisms expressing styrene monooxygenase (SMO) has been studied. Styrene and indole are structurally very similar, and thus we looked at a variety of styrenedegrading strains for indole transformation to indigo. Two strains, Pseudomonas putida S12 and CA-3, gave a blue color on solid media when grown in the presence of indole. Indole induces its own transformation on solid media but is a poor inducer in liquid media. Styrene is the best inducer of indole transformation in both strains. Arginine represses styrene consumption and indigo formation rates in P. putida S12 compared to phenylacetic acid-grown cells, while the opposite effect is seen for P. putida CA-3. Characterization of an SMOand styrene oxide isomerase (SOI)-negative transposon mutant of P. putida CA-3 and an SOI-negative N-methyl-N*-nitro-N-nitrosoguanidine mutant of P. putida S12 reveals the involvement of both SMO and SOI in indole transformation to indigo. Both strains stoichiometrically produce high-purity indigo from indole.read more
Citations
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DNA shuffling method for generating highly recombined genes and evolved enzymes.
Wayne Coco,William E. Levinson,Michael J. Crist,Harm J. Hektor,Aldis Darzins,Philip T. Pienkos,Charles H. Squires,Daniel J. Monticello +7 more
TL;DR: This method represents a conceptually distinct and improved alternative to sexual PCR for gene family shuffling and is demonstrated by molecular breeding of a monooxygenase for increased rate and extent of biodesulfurization on complex substrates, as well as for 20-fold faster conversion of a nonnatural substrate.
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Complete Sequence of a 184-Kilobase Catabolic Plasmid from Sphingomonas aromaticivorans F199
Margaret F. Romine,Lisa C. Stillwell,Kwong Kwok Wong,Sarah J. Thurston,Ellen C. Sisk,Christoph Wilhelm Sensen,Terry Gaasterland,Terry Gaasterland,James K. Fredrickson,Jeffrey D. Saffer +9 more
TL;DR: The complete 184,457-bp sequence of the aromatic catabolic plasmid, pNL1, from Sphingomonas aromaticivorans F199 has been determined, and several genes associated with integration and recombination were identified in the replication region, suggesting that pNL 1 is able to undergo integration and excision events with the chromosome and/or other portions of the plasmids.
Journal ArticleDOI
SigmaS-dependent gene expression at the onset of stationary phase in Escherichia coli: function of sigmaS-dependent genes and identification of their promoter sequences.
TL;DR: Genes involved in protein biosynthesis, encoding the ribosome-associated protein RpsV (sra) and the initiation factor IF-1 (infA), were induced in an rpoS-dependent fashion and significant fractions of these promoters carry sequence features specific for Esigma(S) recognition of the -10 region, thus supporting the TGN(0-2)C(C/T)ATA( C/A)T consensus sequence recently proposed.
Journal ArticleDOI
Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase.
TL;DR: Upon expression in Escherichia coli, some of these evolved genes exhibited enhanced degradation capacity, not only for PCB and related biphenyl compounds, but for single aromatic hydrocarbons such as benzene and toluene, which are poor substrates for the original BP Dox.
Journal ArticleDOI
Towards a Biocatalyst for (S)-Styrene Oxide Production: Characterization of the Styrene Degradation Pathway of Pseudomonas sp. Strain VLB120
TL;DR: To assess the usefulness of the enzymes for the production of enantiomerically pure styrene oxide, the enantiospecificities of the reactions involved were investigated and confirmed a probable function as a phenylacetaldehyde dehydrogenase.
References
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TL;DR: A pathway for styrene degradation via styrene oxide and phenylacetaldehyde to phenylacetic acid is proposed.
Journal ArticleDOI
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
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TL;DR: In this article, a gene cluster from Pseudomonas mendocina KR1 that determines the offO activity was cloned and characterized, and it was tentatively concluded that the tmoABCDE genes encoded structural polypeptides of the T4MO enzyme system.