A
Alfred Pühler
Researcher at Bielefeld University
Publications - 662
Citations - 49153
Alfred Pühler is an academic researcher from Bielefeld University. The author has contributed to research in topics: Gene & Plasmid. The author has an hindex of 102, co-authored 658 publications receiving 45871 citations. Previous affiliations of Alfred Pühler include Facultad de Ciencias Exactas y Naturales & National University of La Plata.
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Journal ArticleDOI
A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria
TL;DR: In this paper, a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli was developed, which can utilize any gram negative bacterium as a recipient for conjugative DNA transfer.
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Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum
TL;DR: Small mobilizable vectors based on the Escherichia coli plasmids pK18 and pK19 can be transferred by RP4-mediated conjugation into a wide range of Gram- and Gram+ bacteria, and should facilitate gene disruption and allelic exchange by homologous recombination.
Journal ArticleDOI
The composite genome of the legume symbiont Sinorhizobium meliloti.
Francis Galibert,Turlough M. Finan,Sharon R. Long,Sharon R. Long,Alfred Pühler,Pia Abola,Frédéric Ampe,Frédérique Barloy-Hubler,Melanie J. Barnett,Anke Becker,P. Boistard,G. Bothe,Marc Boutry,Leah Bowser,Jens Buhrmester,Edouard Cadieu,Delphine Capela,Delphine Capela,Patrick S. G. Chain,Alison Cowie,Ronald W. Davis,Stéphane Dréano,Nancy A. Federspiel,Robert F. Fisher,Stéphanie Gloux,Thérèse Godrie,André Goffeau,Brian Golding,Jérôme Gouzy,Mani Gurjal,Ismael Hernández-Lucas,Andrea Hong,Lucas Huizar,Richard W. Hyman,Ted Jones,Daniel Kahn,Michael L. Kahn,Sue Kalman,David H. Keating,David H. Keating,Ernö Kiss,Caridad Komp,Valerie Lelaure,David Masuy,Curtis J. Palm,Melicent C. Peck,Thomas M. Pohl,Daniel Portetelle,Bénédicte Purnelle,Uwe Ramsperger,Raymond Surzycki,Patricia Thebault,Micheline Vandenbol,Frank-J. Vorhölter,Stefan Weidner,Derek H. Wells,Kim Wong,Kuo-Chen Yeh,Kuo-Chen Yeh,Jacques Batut +59 more
TL;DR: The annotated DNA sequence of the α-proteobacteriumSinorhizobium meliloti, the symbiont of alfalfa, is presented, indicating that all three elements contribute, in varying degrees, to symbiosis and reveals how this genome may have emerged during evolution.
Journal ArticleDOI
Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment.
Jonas Schulte-Schrepping,Nico Reusch,Daniela Paclik,Kevin Baßler,Stephan Schlickeiser,Bowen Zhang,Benjamin Krämer,Tobias Krammer,Sophia Brumhard,Lorenzo Bonaguro,Elena De Domenico,Daniel Wendisch,Martin Grasshoff,Theodore S. Kapellos,Michael Beckstette,Tal Pecht,Adem Saglam,Oliver Dietrich,Henrik E. Mei,Axel Schulz,Claudia Conrad,Désirée Kunkel,Ehsan Vafadarnejad,Cheng-Jian Xu,Cheng-Jian Xu,Arik Horne,Miriam Herbert,Anna Drews,Charlotte Thibeault,Moritz Pfeiffer,Stefan Hippenstiel,Andreas C. Hocke,Holger Müller-Redetzky,Katrin-Moira Heim,Felix Machleidt,Alexander Uhrig,Laure Bosquillon de Jarcy,Linda Jürgens,Miriam Stegemann,Christoph R. Glösenkamp,Hans-Dieter Volk,Christine Goffinet,Markus Landthaler,Emanuel Wyler,Philipp Georg,Maria Schneider,Chantip Dang-Heine,Nick Neuwinger,Kai Kappert,Rudolf Tauber,Victor M. Corman,Jan Raabe,Kim Melanie Kaiser,Michael To Vinh,Gereon Rieke,Christian Meisel,Thomas Ulas,Matthias Becker,Robert Geffers,Martin Witzenrath,Christian Drosten,Norbert Suttorp,Christof von Kalle,Florian Kurth,Florian Kurth,Florian Kurth,Kristian Händler,Joachim L. Schultze,Joachim L. Schultze,Anna C. Aschenbrenner,Anna C. Aschenbrenner,Yang Li,Yang Li,Jacob Nattermann,Birgit Sawitzki,Antoine-Emmanuel Saliba,Leif E. Sander,Angel Angelov,Robert Bals,Alexander Bartholomäus,Anke Becker,Daniela Bezdan,Ezio Bonifacio,Peer Bork,Thomas Clavel,Maria Colomé-Tatché,Andreas Diefenbach,Alexander T. Dilthey,Nicole Fischer,Konrad U. Förstner,Julia-Stefanie Frick,Julien Gagneur,Alexander Goesmann,Torsten Hain,Michael Hummel,Stefan Janssen,Jörn Kalinowski,René Kallies,Birte Kehr,Andreas Keller,Sarah Kim-Hellmuth,Christoph Klein,Oliver Kohlbacher,Jan O. Korbel,Ingo Kurth,Kerstin U. Ludwig,Oliwia Makarewicz,Manja Marz,Alice C. McHardy,Christian Mertes,Markus M. Nöthen,Peter Nürnberg,Uwe Ohler,Stephan Ossowski,Jörg Overmann,Silke Peter,Klaus Pfeffer,Anna R. Poetsch,Alfred Pühler,Nikolaus Rajewsky,Markus Ralser,Olaf Rieß,Stephan Ripke,Ulisses Nunes da Rocha,Philip Rosenstiel,Philipp H. Schiffer,Eva-Christina Schulte,Alexander Sczyrba,Oliver Stegle,Jens Stoye,Fabian J. Theis,Janne Vehreschild,Jörg Vogel,Max von Kleist,Andreas Walker,Jörn Walter,Dagmar Wieczorek,John Ziebuhr +137 more
TL;DR: This study provides detailed insights into the systemic immune response to SARS-CoV-2 infection and it reveals profound alterations in the myeloid cell compartment associated with severe COVID-19.
Journal ArticleDOI
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l-aspartate-derived amino acids and vitamins
Jörn Kalinowski,Brigitte Bathe,Daniela Bartels,Nicole Bischoff,Michael Bott,Andreas Burkovski,Nicole Dusch,Lothar Eggeling,Bernhard J. Eikmanns,Lars Gaigalat,Alexander Goesmann,Michael Hartmann,Klaus Huthmacher,Reinhard Krämer,Burkhard Linke,Alice C. McHardy,Folker Meyer,Bettina Möckel,Walter Pfefferle,Alfred Pühler,Daniel Rey,Christian Rückert,Oliver Rupp,Hermann Sahm,Volker F. Wendisch,Iris Wiegräbe,Andreas Tauch +26 more
TL;DR: These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil.