Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil
TLDR
The chances of recovering T. canis eggs from samples using flotation solutions can be increased by washing of soil twice using distilled water, and resuspension of sediment, and the sieving procedure can drastically reduce the number of eggs.Abstract:
The purpose of this study was to evaluate the influence of variables in a flotation technique for the recovery of Toxocara canis eggs from soil. The trials were done under standardized conditions on one gram of previously sterilized soil samples contaminated with 200 eggs of T. canis. The following variables were evaluated in serial steps: sieving; type of wash; time of stirring; resuspension of sediment; solution flotation. Centrifuge-flotation in sodium nitrate (d = 1.20 g/cm(3)) was adopted as an initial technique, using Tween 80 (0.2%) and decinormal sodium hydroxide as solutions for washing the samples. Ten tests were done to compare the variables, using counting in triplicate. The sieving of the material reduced significantly the recovery of eggs (p < 0.001) and the number of eggs recovered was higher when the sediment was resuspended (p < 0.05). After standardization, flotation solutions sodium chloride, zinc sulfate, sodium dichromate, magnesium sulfate, and sodium nitrate (d = 1.20g/cm(3)) were compared. The best results were obtained by using zinc sulfate solution. In conclusion, the chances of recovering T. canis eggs from samples using flotation solutions can be increased by washing of soil twice using distilled water, and resuspension of sediment. On the other hand, the sieving procedure can drastically reduce the number of eggs.read more
Citations
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Detection and quantification of soil-transmitted helminths in environmental samples: A review of current state-of-the-art and future perspectives
TL;DR: It may be concluded that there is a need to comparatively assess the performance of different methods to determine their applicability in different settings as well as for use with different sample matrices.
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Methods for Quantification of Soil-Transmitted Helminths in Environmental Media: Current Techniques and Recent Advances
TL;DR: It is concluded that methods for sampling and recovering STHs require substantial advances to provide reliable measurements for STH control and recent innovations in the use of automated image identification and developments in molecular genetic assays offer considerable promise for improving quantification and viability assessment.
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Environmental contamination with Toxocara spp. eggs in public parks and playground sandpits of Greater Lisbon, Portugal
David Otero,Ana Margarida Alho,Rolf Nijsse,Jeroen Roelfsema,Paul Overgaauw,Luís Madeira de Carvalho +5 more
TL;DR: Public parks and playground sandpits in the Lisbon area were found to be heavily contaminated with T. cati eggs, representing a serious menace to public health as the studied areas represent common places where people of all ages, particularly children, recreate.
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Urban Dog Parks as Sources of Canine Parasites: Contamination Rates and Pet Owner Behaviours in Lisbon, Portugal.
Ana Filipa Ferreira,Ana Margarida Alho,David Otero,Lídia Gomes,Rolf Nijsse,Paul Overgaauw,Luís Madeira de Carvalho +6 more
TL;DR: The results highlight the potential of these parks as a source of transmission for canine parasites, including some with zoonotic potential, and public awareness and effective preventive measures should be promoted to minimise the health-risk impact to both animals and humans.
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A comparison of Toxocara canis embryonation under controlled conditions in soil and hair.
J. Devoy Keegan,Celia V. Holland +1 more
TL;DR: It is demonstrated that unembryonated T. canis eggs are capable of development in hair under controlled conditions, lower than that observed in soil, but remains biologically significant in terms of the overall numbers of potentially infective embryonated eggs present.
References
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