Open Access
Inter- and Intra-Island Genetic Diversity in Adriatic Populations of Croatia: Implications for Studying Complex Diseases in Isolated Populations
Irena Martinović Klarić,Li Jin,Ranajit Chakraborty,Ranjan Deka,Lovorka Barać,Marijana Peričić,Nina Smolej Narančić,Igor Rudan,Branka Janićijević,Pavao Rudan +9 more
Reads0
Chats0
TLDR
The high mutation rate at these STR loci diluted the effect of small effective size in producing correlation of alleles within individuals, and the non-significant gene differentiation between villages within the islands is an advantage for disease-gene association studies using microsatellite loci.Abstract:
Adriatic populations residing in over 7 islands of Croatia have been extensively studied for over the past 30 years. Linguistic, social, physical and biological anthropological data indicate that the island populations have a considerable degree of genetic isolation from each other. Within the islands as well, some degree of isolation exists between villages of several islands. To examine the effects of such genetic isolation on patterns of gene diversity at microsatellite loci, this investigation studied the coefficient of gene differentiation (GST) between villages within islands as well as between islands using allele frequency data at 9 short tandem repeat loci (D3S1358, vWA, FGA, THO1, TPOX, CSF1PO, D5S818, D13S317, and D7S820) in 31 villages of 4 islands (Hvar, Krk, Brac, and Korcula). Overall, none of the villages exhibits any significant departure from Hardy-Weinberg expectations of genotype frequencies at these loci. Decomposition of heterozygosity (H) as well as allele size variance (V) indicated significant inter-island gene differentiation (GST based on H = 0.0032, P < 10-4 ; GST based on V = 0.0036, P = 0.007), while the between villages within island component of genetic variation remain non-significant (GST(H) = 0.0029, P = 0.073 ; GST(V) = 0.0018, P = 0.315). The coefficient of coancestry (q) for the sampled unrelated individuals vary from village to village, ranging from 0.0 to 0.022, with an average of 0.0098. Comparison of estimates of inbreeding coefficient (F) based on genealogical data from these villages indicates that while q and F values are moderately correlated across villages, q is comparatively smaller (nearly one-half) than F. In other words, the high mutation rate at these STR loci diluted the effect of small effective size in producing correlation of alleles within individuals. The non-significant gene differentiation between villages within the islands is an advantage for disease-gene association studies using microsatellite loci, since data from several villages within each island may be pooled to enlarge the sample size without introducing complications of population substructure effects on the association results. (Researh supported by NIH grants GM 41399, GM 52601, and GM 58545).read more
Citations
More filters
Journal ArticleDOI
Inbreeding and the Genetic Complexity of Human Hypertension
Igor Rudan,Nina Smolej-Narančić,Harry Campbell,Andrew D. Carothers,Alan F. Wright,Branka Janićijević,Pavao Rudan +6 more
TL;DR: It is inferred that recent inbreeding accounts for 36% of all hypertension in this population, which may be substantial since, although inbreeding is declining in Western societies, an estimated 1 billion people globally show rates of consanguineous marriages >20%.
Journal ArticleDOI
3000 years of solitude: extreme differentiation in the island isolates of Dalmatia, Croatia.
Veronique Vitart,Zrinka Biloglav,Caroline Hayward,Branka Janićijević,Nina Smolej-Narančić,Lovorka Barać,Marijana Peričić,Irena Martinović Klarić,Tatjana Škarić-Jurić,Maja Barbalić,Ozren Polasek,Ozren Polasek,Ivana Kolcic,Andrew D. Carothers,Pavao Rudan,Nicholas D. Hastie,Alan F. Wright,Harry Campbell,Igor Rudan,Igor Rudan +19 more
TL;DR: A very high level of differentiation is found between most of these island communities based on Wright's fixation indexes, even within the same island, and strong isolation and endogamy are likely to be the main forces maintaining this highly structured overall population.
Related Papers (5)
Genetic Variation in a Widespread, Epiphytic Orchid: Where is the Evolutionary Potential?
James D. Ackerman,Sheila Ward +1 more
A Comprehensive Study of Genic Variation in Natural Populations of Drosophila melanogaster. II. Estimates of Heterozygosity and Patterns of Geographic Differentiation
Rama S. Singh,Lorenz R. Rhomberg +1 more