Journal ArticleDOI
Isolation and characterization of individual functionally reactive cytotoxic T lymphocytes: conjugation, killing and recycling at the single cell level
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TLDR
Direct evidence obtained at the single cell level shows that a single effector lymphocyte is required and sufficient for the destruction of a single target cell and that killer cells which have been responsible for the lysis of a given target cell can lyse a second and even a third time.Abstract:
Isolation and characterization of individual functionally reactive cytotoxic T lymphocytes have been achieved. Peritoneal exudate cytotoxic lymphocytes were obtained from BALB/c mice injected with EL4 tumor cells. Lymphocyte tumor cell conjugation was promoted by centrifugation. Individual conjugates comprised of one lymphocyte bound to one tumor cell were isolated with a micropipette. The ultrastructure of isolated killer lymphocytes and the lysis of conjugated target cells were analyzed. The cytotoxic lymphocytes are small cells with an indented nucleus which is poor in peripheral chromatin and rich in rough nuclear sap. The cytoplasm contains one-membrane-bound lysosome-like granules and clusters of ribosomes, but no rough endoplasmatic reticulum. The Golgi apparatus is well developed. Direct evidence obtained at the single cell level shows that a single effector lymphocyte is required and sufficient for the destruction of a single target cell and that killer cells which have been responsible for the lysis of a given target cell can lyse a second and even a third time.read more
Citations
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Journal ArticleDOI
The Immunological Synapse of CTL Contains a Secretory Domain and Membrane Bridges
TL;DR: It is shown that although target cell death occurs within 5 min of CTL-target cell contact, an immunological synapse similar to that seen in CD4 cells rapidly forms in CTL, with a ring of adhesion proteins surrounding an inner signaling molecule domain.
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Role of lymphocyte adhesion receptors in transient interactions and cell locomotion.
TL;DR: It is proposed that integrin avidity cycles may have an important role in the interconnected phenomena of locomotion, initial cell- cell adhesion, and cell-cell deadhesion.
Journal ArticleDOI
The Binding and Lysis of Target Cells by Cytotoxic Lymphocytes: Molecular and Cellular Aspects
TL;DR: Two proposed mechanisms of lymphotoxicity currently appear to be valid: a membranolytic one initiated by the formation of pores in target cell membranes by secreted molecules of lymphocyte origin, such as perforin and granzymes, and a nonsecretoryOne initiated by receptor-mediated triggering of apoptosis-inducing target cell surface molecules, but not involving the secretion of pore-forming agents and granZymes.
Journal ArticleDOI
Spatial relationships of microtubule-organizing centers and the contact area of cytotoxic T lymphocytes and target cells.
TL;DR: It is proposed that the CTL plasma membrane proximal to the MTOC is particularly active in forming stable intercellular contacts, resulting in CTL-TC conjugation, and that subsequent modulation of the microtubular system of the C TL may be related to the cytolytic response and to detachment of the effector cell.
Journal ArticleDOI
Mononuclear cells in myopathies: Quantitation of functionally distinct subsets, recognition of antigen-specific cell-mediated cytotoxicity in some diseases, and implications for the pathogenesis of the different inflammatory myopathies
Andrew G. Engel,Kiichi Arahata +1 more
TL;DR: Monoclonal antibodies reactive for B cells,T cells, T-cell subsets, killer (K) and natural killer (NK) cells, and the Ia antigen were used to analyze mononuclear cell subsets in scleroderma, dermatomyositis, polymyositis), inclusion body myositis (IBM), Duchenne dystrophy (DD), and normal muscle.
References
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Book ChapterDOI
Cell-Mediated Cytotoxicity, Allograft Rejection, and Tumor Immunity
TL;DR: Specific cell-mediated cytotoxicity in vitro can be divided into three categories according to the nature of the effector cells, which is most often highly specific and requires intimate contact rather than release of diffusible toxic factors.
Journal ArticleDOI
HIGH-RESOLUTION AUTORADIOGRAPHY : I. Methods
TL;DR: In this paper, the authors describe methods used in obtaining high resolution in autoradiography, with special emphasis on the technique of electron microscopic autoregressive imaging, together with control experiments designed to establish the optimum conditions or procedures.
Journal ArticleDOI
High-resolution autoradiography
TL;DR: Methods used in obtaining high resolution in autoradiography, with special emphasis on the technique of electron microscopic autoradography, are described, together with control experiments designed to establish the optimum conditions or procedures.
Journal ArticleDOI
Specific in vitro Cytotoxicity of Thymus-derived Lymphocytes sensitized to Alloantigens
TL;DR: This study tested the effect of anti-θ serum on the cytotoxic activity of spleen cells sensitized to alloantigens, and its effect on alloantsibody-producing cells present in the same spleen cell populations was tested in parallel.
Journal ArticleDOI
Quantitative studies on the behavior of sensitized lymphocytes in vitro : i. relationship of the degree of destruction of homologous target cells to the number of lymphocytes and to the time of contact in culture and consideration of the effects of isoimmune serum.
TL;DR: The cytocidal effect of specifically sensitized lymphoid cells on homologous target cells in culture can be inhibited by small quantities of an imidazole derivative of 6-mercaptopurine (imuran).