Kinetic study of DNA modification by phthalocyanine derivative of the oligonucleotide.
Alexandra A. Kuznetsova,A. A. Chernonosov,Nikita A. Kuznetsov,Vladimir V. Koval,Dmitri G. Knorre,Olga S. Fedorova +5 more
TLDR
This study has investigated the kinetics and thermodynamics of sequence-specific modification of DNA with deoxyribooligonucleotide linked to Co(II)-tetracarboxyphthalocyanine (PtcCo(II)) in the presence of H2O2.Abstract:
Design of chemically modified oligonucleotides for regulation of gene expression has attracted considerable attention over the last decades. One actively pursued approach involves antisense or antigene constructs carrying reactive groups, many of these based on transition metal complexes. The complexes of Co(II) with phthalocyanines are extremely good catalysts of oxidation of organic compounds with molecular oxygen and hydrogen peroxide. In this study, we have investigated the kinetics and thermodynamics of sequence-specific modification of DNA with deoxyribooligonucleotide linked to Co(II)-tetracarboxyphthalocyanine (PtcCo(II)) in the presence of H2O2.read more
Citations
More filters
Journal ArticleDOI
Solid-phase synthesis of azaphthalocyanine-oligonucleotide conjugates and their evaluation as new dark quenchers of fluorescence.
TL;DR: Hydrophobic nonaggregating metal-free azaphthalocyanines of the tetrapyrazinoporphyrazine type were synthesized, characterized, and used for oligonucleotide labeling and quenching efficiency was evaluated in a simple hybridization assay using monolabeled probes.
Journal ArticleDOI
DNA-binding and oxidative properties of cationic phthalocyanines and their dimeric complexes with anionic phthalocyanines covalently linked to oligonucleotides.
TL;DR: It was determined that oxidation of DNA by molecular oxygen catalyzed by complex of Fe(II)-phthalocyanines proceeds with higher rate than in the case of Co(II), but the latter led to a greater extent of target DNA modification.
Journal ArticleDOI
DNA Complexes with Cobalt(II) Phthalocyanine Disodium Disulfonate.
Nina A. Kasyanenko,Roman A. Tikhomirov,Vladimir Bakulev,V. N. Demidov,Elena V. Chikhirzhina,Eugenia B. Moroshkina +5 more
TL;DR: The interaction of cobalt phthalocyanine disodium disulfonate (CoPc) with calf thymus DNA in solutions was investigated by UV/vis spectrophotometry, circular dichroism (CD), and hydrodynamic methods.
Patent
Functionalized fluorine containing phthalocyanine molecules
David Sabatino,Sergiu M. Gorun,Emily Borland,Hemantbhai H. Patel,Pradeepkumar Patel,Erik N. Carrión +5 more
TL;DR: In this article, the fluorine containing phthalocyanine molecules are functionalized with a reactive functional group or at least one cancer-targeting ligand (CTL), which can facilitate more efficient binding and/or internalization to a cancer cell than to a healthy cell.
Journal ArticleDOI
Fe(II) phthalocyanine catalyzed oxidation of dGMP by molecular oxygen
Aleksandra A. Kuznetsova,Lyudmila I. Solovyeva,Oleg L. Kaliya,E. A. Lukyanets,Dmitri G. Knorre,Olga S. Fedorova,Olga S. Fedorova +6 more
TL;DR: It is shown that iron(II)-phthalocyanines are able to catalyze guanosine oxidation by molecular oxygen in the presence of reducing agents such as ascorbic acid and 2-mercaptoethanol.
References
More filters
Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Program DYNAFIT for the Analysis of Enzyme Kinetic Data: Application to HIV Proteinase
TL;DR: A computer program with the code name DYNAFIT was developed for fitting either the initial velocities or the time course of enzyme reactions to an arbitrary molecular mechanism represented symbolically by a set of chemical equations.
Journal ArticleDOI
Oxidative DNA damage and disease: induction, repair and significance
TL;DR: The weight of evidence strongly suggests a link between such damage and the pathogenesis of disease, and the role of 8-OH-dG in disease, although exact roles remain to be elucidated.