Journal ArticleDOI
Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies.
Alois Jungbauer,Rainer Hahn +1 more
TLDR
The pressure drop characteristics, mass transfer properties, scale-up, and applications of monoliths in the context of conventional chromatography media are discussed.About:
This article is published in Journal of Chromatography A.The article was published on 2008-03-14. It has received 218 citations till now. The article focuses on the topics: Monolithic HPLC column & Hydrophilic interaction chromatography.read more
Citations
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Journal ArticleDOI
Porous polymer monoliths: amazingly wide variety of techniques enabling their preparation.
TL;DR: A vast variety of methods that have meanwhile emerged for the preparation of monoliths comprising standard free radical polymerizations, including stable free radicals, atom transfer radical polymerization, and ring-opening metathesis polymerization are presented.
Journal ArticleDOI
High Throughput Isolation and Glycosylation Analysis of IgG–Variability and Heritability of the IgG Glycome in Three Isolated Human Populations
Maja Pučić,Ana Knezevic,Jana Vidič,Barbara Adamczyk,Mislav Novokmet,Ozren Polasek,Olga Gornik,Sandra Supraha-Goreta,Mark R. Wormald,Irma Redzic,Harry Campbell,Alan F. Wright,Nicholas D. Hastie,James F. Wilson,Igor Rudan,Manfred Wuhrer,Pauline M. Rudd,Djuro Josic,Djuro Josic,Gordan Lauc +19 more
TL;DR: The majority of the structural features of the IgG glycome were consistent with previous studies, but sialylation was somewhat higher than reported previously, indicating that the final glycan structures are not a simple result of competing enzymatic activities, but a carefully regulated outcome designed to meet the prevailing physiological needs.
Journal ArticleDOI
Advances and recent trends in the field of monolithic columns for chromatography.
Frantisek Svec,Yongqin Lv +1 more
Journal ArticleDOI
Bioprocess challenges to the isolation and purification of bioactive peptides
TL;DR: By coupling the high throughput and predictive capability of ‘-omics’ to the enhanced convective hydrodynamics of monolithic columns, it becomes feasible, even at preparative scale, to produce BPs that meet the requirements of high purity, potency, and cost-effectiveness.
Journal ArticleDOI
Latest Advances in Cryogel Technology for Biomedical Applications
Adnan Memic,Adnan Memic,Adnan Memic,Thibault Colombani,Loek J. Eggermont,Loek J. Eggermont,Mahboobeh Rezaeeyazdi,Joseph M Steingold,Zachary J. Rogers,Kasturi Joshi Navare,Halimatu S. Mohammed,Sidi A. Bencherif +11 more
TL;DR: In this paper, a review of the methods for synthesis and characterization of Cryogels is provided, highlighting key concepts relevant to cryogels and explaining their unique properties, as well as an in-depth overview of specific technologies and fields where they have been applied.
References
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Book
Diffusion: Mass Transfer in Fluid Systems
TL;DR: An overview of diffusion and separation processes brings unsurpassed, engaging clarity to this complex topic as mentioned in this paper, which is a key part of the undergraduate chemical engineering curriculum and at the core of understanding chemical purification and reaction engineering.
Journal ArticleDOI
A theory of gel filtration and its exeperimental verification
TL;DR: The experimentally determined available volumes in the gel phase for three proteins are approximately the same as the Available volumes in dextran solutions, having the same polymer concentrations as the gels.
Journal ArticleDOI
New designs of macroporous polymers and supports : From separation to biocatalysis
TL;DR: Polymer monoliths containing intricate pore networks can be obtained in any desired shape by a simple molding process and provide unique advantages such as fast kinetics, high reactions, and high throughput.
Journal ArticleDOI
High-Performance Membrane Chromatography. A Novel Method of Protein Separation
TL;DR: In this article, 1 mm thick membranes from macroporous methacrylate polymer provided with functional groups were synthetized and used for protein separation and the results show that the separation is fully comparable with that experienced on a filled column but the advantage of a membrane is up to two orders of magnitude lower pressure during the process and very high loading reaching up to 40 g/m2.
Journal ArticleDOI
Silica gel-based monoliths prepared by the sol-gel method: facts and figures.
TL;DR: The purpose of this paper is to deal with the problems associated with silica based monoliths, including cracking and the shrinkage of the bed during drying.
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Porous polymer monoliths: amazingly wide variety of techniques enabling their preparation.
Monoliths for fast bioseparation and bioconversion and their applications in biotechnology
Alois Jungbauer,Rainer Hahn +1 more