Journal ArticleDOI
Application of monoliths for plasmid DNA purification: Development and transfer to production
TLDR
It could be shown, that up-scaling to the production scale using 800 ml CIM Convective Interaction Media radial flow monoliths is possible under low pressure conditions and CIM DEAE was successfully implemented as intermediate step of the cGMP pDNA manufacturing process.About:
This article is published in Journal of Chromatography A.The article was published on 2005-02-11. It has received 156 citations till now. The article focuses on the topics: Monolithic HPLC column.read more
Citations
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Journal ArticleDOI
Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies.
Alois Jungbauer,Rainer Hahn +1 more
TL;DR: The pressure drop characteristics, mass transfer properties, scale-up, and applications of monoliths in the context of conventional chromatography media are discussed.
Journal ArticleDOI
Latest Advances in Cryogel Technology for Biomedical Applications
Adnan Memic,Adnan Memic,Adnan Memic,Thibault Colombani,Loek J. Eggermont,Loek J. Eggermont,Mahboobeh Rezaeeyazdi,Joseph M Steingold,Zachary J. Rogers,Kasturi Joshi Navare,Halimatu S. Mohammed,Sidi A. Bencherif +11 more
TL;DR: In this paper, a review of the methods for synthesis and characterization of Cryogels is provided, highlighting key concepts relevant to cryogels and explaining their unique properties, as well as an in-depth overview of specific technologies and fields where they have been applied.
Journal ArticleDOI
Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA.
TL;DR: Diverse affinity applications and their advantages and disadvantages are discussed, as well as the most significant results and improvements in the challenging task of purifying plasmids.
Journal ArticleDOI
Applications of polymethacrylate-based monoliths in high-performance liquid chromatography.
E. G. Vlakh,Tatiana B. Tennikova +1 more
TL;DR: In this review, the applications of polymethacrylate-based monolithic columns are summarized for the separation, purification and analysis of low and high molecular mass compounds in the different HPLC formats, including micro- and large-scale HPLC modes.
Journal ArticleDOI
Tools for analyzing the phosphoproteome and other phosphorylated biomolecules: a review.
TL;DR: This review will focus on sample preparation techniques in the field of phosphoproteomics, but also highlight recent advancements for the analysis of other phosphorylated biomolecules.
References
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Journal ArticleDOI
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
Book
DNA Structure and Function
TL;DR: This chapter discusses the structure and function of DNA, which is a right handed helix of two individual antiparallel DNA strands that is the source of all intrinsic genetic information.
Journal ArticleDOI
Nonviral Gene Therapy: The Promise of Genes as Pharmaceutical Products
TL;DR: Future advances in nonviral systems will be based on an emerging appreciation of the biological constraints on the fate and function of DNA within the body and within the cell.
Journal ArticleDOI
Behavior of Supercoiled DNA
TL;DR: The molecule's degree of supercoiling could therefore be quantitatively controlled and monitored, and tethered-particle motion analysis allowed us to measure the stretching force acting on the DNA.
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Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies.
Alois Jungbauer,Rainer Hahn +1 more