Journal ArticleDOI
Lectins: a primer for histochemists and cell biologists.
Joachim C. Manning,Antonio Romero,Felix A. Habermann,Gabriel García Caballero,Herbert Kaltner,Hans-Joachim Gabius +5 more
TLDR
The functional pairing with physiological counterreceptors is involved in a wide range of cellular activities from cell adhesion, glycoconjugate trafficking to growth regulation and lets lectins act as sensors/effectors in host defense.Abstract:
An experimental observation on selecting binding partners underlies the introduction of the term 'lectin'. Agglutination of erythrocytes depending on their blood-group status revealed the presence of activities in plant extracts that act in an epitope-specific manner like antibodies. As it turned out, their binding partners on the cell surface are carbohydrates of glycoconjugates. By definition, lectins are glycan-specific (mono- or oligosaccharides presented by glycoconjugates or polysaccharides) receptors, distinguished from antibodies, from enzymes using carbohydrates as substrates and from transporters of free saccharides. They are ubiquitous in Nature and structurally widely diversified. More than a dozen types of folding pattern have evolved for proteins that bind glycans. Used as tool, this capacity facilitates versatile mapping of glycan presence so that plant/fungal and also animal/human lectins have found a broad spectrum of biomedical applications. The functional pairing with physiological counterreceptors is involved in a wide range of cellular activities from cell adhesion, glycoconjugate trafficking to growth regulation and lets lectins act as sensors/effectors in host defense.read more
Citations
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Journal ArticleDOI
C-type lectins: their network and roles in pathogen recognition and immunity.
TL;DR: Targeting CTLs, key players in pathogen recognition and innate immunity, may be a promising strategy for cell-specific delivery of drugs or vaccine antigens and to modulate immune responses.
Journal ArticleDOI
Sialylation of N-glycans: mechanism, cellular compartmentalization and function.
Gaurang P. Bhide,Karen J. Colley +1 more
TL;DR: This review approaches the sialylation of N-glycans from three perspectives, commenting on the role of sialic acid-binding proteins including viral hemagglutinins, Siglecs and selectins and the roles of β1-integrin and Fas receptor N- Glycans in cancer cell survival and drug resistance.
Journal ArticleDOI
The medicinal and pharmaceutical importance of Dendrobium species
TL;DR: This mini-review discusses the latest advances in what is known about the medicinal and pharmaceutical properties of members of the Dendrobium genus and explores how biotechnology can serve as a conduit to mass propagate valuable germplasm for sustainable exploration for the pharmaceutical industry.
Journal ArticleDOI
Eukaryotic protein glycosylation: a primer for histochemists and cell biologists.
TL;DR: Following this educational survey, examples where known biological function is related to the glycan structures carried by proteins are given and mucins and their glycosylation patterns are considered as instructive proof-of-principle case.
Journal ArticleDOI
Galectins: their network and roles in immunity/tumor growth control
Herbert Kaltner,Stefan Toegel,Gabriel García Caballero,Joachim C. Manning,Robert W. Ledeen,Hans-Joachim Gabius +5 more
TL;DR: To delineate the factors that underlie the specificity of a galectin for its counterreceptor(s) in the cellular context and the details of structure–activity relationships by comparatively analyzing natural and rationally engineered proteins is the main challenge for ongoing research.
References
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Journal Article
Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes.
TL;DR: The studies suggest that the mitogenic action of PHA does not involve mitosis per se but rather the stage preceding mitosis—the alteration of circulating monocytes and large lymphocytes to a state wherein they are capable of division.
Journal ArticleDOI
Ribosome-inactivating proteins from plants
Book ChapterDOI
2 – Isolation, Physicochemical Characterization, and Carbohydrate-Binding Specificity of Lectins
Journal ArticleDOI
The Covalent and Three-Dimensional Structure of Concanavalin A
Gerald M. Edelman,Bruce A. Cunningham,George N. Reeke,Joseph W. Becker,Myron J. Waxdal,John L. Wang +5 more
TL;DR: The tentative amino-acid sequence and three-dimensional structure of the lectin concanavalin A have been determined and the point of cleavage for the formation of the naturally occurring fragments A(1) and A(2), have been tentatively assigned.