Localization of antigen in tissue cells ii. improvements in a method for the detection of antigen by means of fluorescent antibody
Albert H. Coons,Melvin H. Kaplan +1 more
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Improvements in a method for the specific microscopic localization of antigen in tissue cells are described and two isomeric series derived from nitrofluorescein are described.Abstract:
Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.read more
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A simple method of reducing the fading of immunofluorescence during microscopy
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Technical aspects of immunohistochemistry.
TL;DR: An overview of technical aspects of immunohistochemistry, including those related to antibodies and Ags, fixation, AR, detection methods, background, and troubleshooting are provided.
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Protein microchips: use for immunoassay and enzymatic reactions.
Pavel Arenkov,Alexander Kukhtin,Anne Gemmell,Sergey Voloshchuk,Valentina Vladimirovna Chupeeva,Andrei D. Mirzabekov,Andrei D. Mirzabekov +6 more
TL;DR: Protein microchips were used in immunoassays for detection of antigens or antibodies, as well as to carry out enzymatic reactions and to measure their kinetics in the absence or presence of an inhibitor.
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Radioimmunoassay for tumor antigen of human cervical squamous cell carcinoma
Hiroshi Kato,Tadashi Torigoe +1 more
TL;DR: A heterologous antiserum for human cervical squamous cell carcinoma was prepared and specificity determined by Ouchterlony immunodiffusion and immunofluoresence studies.
References
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Localization of antigen in tissue cells iii. cellular distribution of pneumococcal polysaccharides types ii and iii in the mouse
TL;DR: The cytological distribution of the pneumococcal polysaccharides, types II and III, was followed in the tissues of the mouse, and the significance of its fixation in relation to its antigenicity and possible toxicity in mice is discussed.
Journal ArticleDOI
The Zone of Localization of Anti-Mouse-Kidney Serum as Determined by Radioautographs.
Journal Article
The Permeability of the Renal Glomeruli of Several Mammalian Species to Labelled Proteins.
Journal ArticleDOI
Phenyl isocyanate protein compounds and their immunological properties.
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