Journal ArticleDOI
Loci of Action of Regulators of Aldosterone Biosynthesis in Isolated Glomerulosa Cells
Greti Aguilera,K. J. Catt +1 more
TLDR
The effects of angiotensin II (All), ACTH, and potassium on early and late steps of aldosterone biosynthesis were studied in collagenase-dispersed cells from rat adrenal capsules and dog zona glomerulosa to measure the formation of pregnenolone and later steps of biosynthetic conversion in the absence of endogenous precursors.Abstract:
The effects of angiotensin II (All), ACTH, and potassium on early and late steps of aldosterone biosynthesis were studied in collagenase-dispersed cells from rat adrenal capsules and dog zona glomerulosa. Isolated cells were incubated with a cyanoketone derivative (WIN 19,578) to isolate the nearly and late portions of the biosynthetic pathway. In this way, the formation of pregnenolone and later steps of biosynthetic conversion in the absence of endogenous precursors could be measured independently during stimulation by the three regulators. During incubation for 3 h with 10 nM All, 10 nM ACTH, or 15 mM potassium, marked stimulation of pregnenolone production was observed in glomerulosa cells of both species. In rat glomerulosa cells, each of the three regulators also stimulated conversion of exogenous corticosterone to aldosterone. In dog glomerulosa cells, All and potassium, but not ACTH, stimulated the conversion of corticosterone to aldosterone. In rat glomerulosa cells, aldosterone production from e...read more
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Journal ArticleDOI
Role of calcium and cAMP in the action of adrenocorticotropin on aldosterone secretion.
TL;DR: It is indicated that ACTH stimulates calcium influx, that calcium potentiates ACTH-induced but not forskolin-induced cAMP generation, and that Ca2+ and cAMP act as synarchic messengers in ACTh-mediated aldosterone secretion.
Journal ArticleDOI
Dependence of Aldosterone Stimulation in Adrenal Glomerulosa Cells on Calcium Uptake: Effects of Lanthanum and Verapamil
John L. Fakunding,Kevin J. Catt +1 more
TL;DR: Results provide further evidence that angiotensin II and potassium increase aldosterone production in adrenal glomerulosa cells through a calcium-dependent mechanism, and indicate that calcium uptake is an essential requirement for the stimulation of ald testosterone production by these two effectors.
Journal ArticleDOI
Heparin-induced aldosterone suppression and hyperkalemia
TL;DR: In this paper, the effects of aldosterone inhibition on the number and affinity of the angiotensin-II receptors in the zona glomerulosa were investigated.
Journal ArticleDOI
Origin of aldosterone in the rat heart
TL;DR: The level of aldosterone content in the healthy rat heart in vivo is significantly lower than that reported elsewhere and reflects plasma levels in intact rats.
References
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A Radioimmunoassay for Cortisol in Plasma and Urine
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Journal ArticleDOI
In vitro effects of ACTH, angiotensins, serotonin and potassium on steroid output and conversion of corticosterone to aldosterone by isolated adrenal cells.
R. Haning,James F. Tait +1 more
TL;DR: Microscopic examination and consideration of steroid outputs suggest that preparations from decapsulated glands consist of zona fasciculata-reticularis cells, while those from capsular glands consist mainly of zzon glomerulosa cells with some zzon fascicULata contamination.
Journal ArticleDOI
A Radioimmunoassay for Aldosterone in Human Peripheral Plasma Including a Comparison of Alternate Techniques
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Journal ArticleDOI
The Mechanism of Action of Adrenocorticotropic Hormone THE ROLE OF MITOCHONDRIAL CHOLESTEROL ACCUMULATION IN THE REGULATION OF STEROIDOGENESIS
TL;DR: The results suggest that ACTH and its mediator cyclic AMP stimulate steroidogenesis by regulating the mitochondrial precursor pool of cholesterol, rather than through a direct effect on the mitochondrial enzyme system that transforms cholesterol to Δ5-pregnenolone.
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In vitro effects of ACTH, angiotensins, serotonin and potassium on steroid output and conversion of corticosterone to aldosterone by isolated adrenal cells.
R. Haning,James F. Tait +1 more