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Manipulation of flavonoid biosynthesis in plants

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TLDR
In this article, the authors present nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of flavonoids biosynthesis in plants.
Abstract
Nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of flavonoid biosynthesis in plants.More particularly, the flavonoid biosynthetic enzyme is selected from the group consisting of chalcone isomerase (CHI), chalcone synthase (CHS), chalcone reductase (CHR), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LCR), flavonoid 3', 5' hydrolase (F3'5'H), flavanone 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), phenylalanine ammonia-olyase (PAL) and vestitone reductase (VR), and functionally active fragments and variants thereof (62) Divided Out of 531985

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Methods and compositions for production of flavonoid and isoflavonoid nutraceuticals

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References
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
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MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations

TL;DR: A computer package, called MAPMAKER, designed specifically for the construction of linkage maps in a number of organisms, including the human and several plants, and it is outlined the mapping strategies that have been used.
Journal ArticleDOI

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer

TL;DR: The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases.
Journal ArticleDOI

Identification of protein coding regions by database similarity search.

TL;DR: The computer program BLASTX performed conceptual translation of a nucleotide query sequence followed by a protein database search in one programmatic step and was characterized as appropriate for use in moderate and large scale sequencing projects at the earliest opportunity, when the data are most prone to containing errors.
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Sequence-determined dna fragments and corresponding polypeptides encoded thereby

TL;DR: In this paper, the authors proposed a method to encode portions of the genome of a plant, and polypeptides encoded thereby, which are useful for expressing a gene product, either as a promoter or as a structural gene or as an UTR or as 3' termination sequence.
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