Showing papers in "Analytical Biochemistry in 1983"

TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.

TL;DR: A highly sensitive fluorometric method for the quantitation of cholesterol, lipid, and other hydroperoxides at the picomole level is described and a 1:1 stoichiometry is observed between the hydroperoxide added and the dichlorofluoroscein produced.

TL;DR: Twenty-eight years ago, Smithies demonstrated that a starch gel could serve as a molecular sieve through which zone electrophoresis of proteins could be carried out and this procedure is now one of the most widely used analytical and preparative tools in cellular and molecular biology.

TL;DR: Improved assay method for measuring myeloperoxidase (MPO) based on work of Andrews and Krinsky using tetramethylbenzidine a noncarcinogenic substrate was 34 times more sensitive than the DMB method.

TL;DR: This stain proved to be a useful adjunct to the enzyme-linked immunoelectrotransfer blot technique and is more sensitive than Coomassie blue, amido black, and fast green stains and is simple to use.

TL;DR: A flexible and convenient computational method for the simulation of kinetic progress curves has been developed and adheres closely with the ANSI 1966 standard, so as to make it maximally portable and machine independent.

TL;DR: For muscarinic, adenosine, alpha 2, dopamine, and estradiol receptors, specific binding measured by the PEI-filter technique was 84-110% of specificbinding measured by gel filtration, demonstrating that the technique gave almost quantitative recovery of bound ligand.

TL;DR: A procedure for quick and simple elution of DNA from agarose gels is presented, useful for rapid preparation of specifically end-labeled DNA fragments and may also be utilized for any other preparative applications.

TL;DR: A method for producing random subclones using sonication to fragment the DNA is presented and under some conditions sonication is shown to shear A + T-rich sequences preferentially, although under most conditions it will create a random subclone library.

TL;DR: Details are provided for a reproducible procedure for determination of labile sulfide in iron-sulfur (Fe-S) proteins in the range of 1 to 3 nmol and analyses are presented on the most stable Fe-S protein so far reported.

TL;DR: This simple, one-step DNA extraction procedure can be utilized in conjunction with Hoechst reagent to obtain quantitative estimates of DNA levels in cell or tissue extracts to eliminate the need for enzyme treatment or exposure to high salt solutions.

TL;DR: From the correlation of the gel bands with 12 isolated polypeptides from which the complete amino acid sequence is known, it is concluded that mammalian cytochrome c oxidase consists of 13 different polypeptin which can all be separated by the described procedure.

TL;DR: A convenient method for mounting slab gels for photography, scanning, and long-term storage has been developed and an improved silver-stain technique which does not require photographic fixers in order to achieve low-level background staining is described.

TL;DR: A solution hybridization assay in which the single-stranded cDNA is used to quantitate mRNA in total nucleic acid samples are described, which can be used to measure RNAs complementary to any cloned DNA sequence.

TL;DR: Tinitrobenzenesulfonic acid titration studies demonstrated that epsilon-amino groups of lysine residues are modified by the coupling of carbonyldiimidazole-activated PEG to proteins.

TL;DR: A very sensitive method for the detection of antigen-antibody complexes on nitrocellulose paper immunoblots is described and the improved of this method is the result of the use of the detergent Tween 20 in blocking the nonspecific binding of the antibodies to the nitrocells.

TL;DR: A synthesis of the N-hydroxysuccinimide ester of S-acetylthioacetic acid is described, which has advantages over the currently available reagents used to introduce sulfhydryl groups into a variety of proteins.

TL;DR: Bands that have cellulolytic activity are visualized after polyacrylamide gel electrophoresis by laying the slab gel on top of a thin sheet of 2% agar containing 0.1% carboxymethylcellulose.

TL;DR: The use of ascorbate as a preserving agent for monoamine metabolites in CSF was not found to be necessary when proper care was exercised in sample handling and storage.

TL;DR: A general semiempirical correction procedure which can be used for absorbance values as high as 5 is described and the importance of choosing the excitation wavelength so as to minimize the necessity for these corrections is emphasized.

TL;DR: The procedure has been used successfully with several dozen isolates of the plant pathogenic fungus Cochliobolus heterostrophus (including both laboratory strains and isolates collected directly from the field), and has been found to be equally suitable for C. carbonum, Neurospora crassa, N. tetrasperma, and Nectria haematococca.

TL;DR: With this assay, Lipidex 1000 appeared to be useful for the delipidation of protein samples at 37 degrees C and for a radiochemical assay of fatty acid-binding by microgram amounts of protein at 0 degree C.

TL;DR: The use of bovine serum albumin as a detergent buffer is described and has been used for assay of Na,K-ATPase activity of membranes from dog kidney, rabbit brain, and electric organ of eel.

TL;DR: This procedure yields lower values for the concentration of malonaldehyde in food samples than the conventional spectrophotometric procedure based on absorbance of the thiobarbituric acid-malonaldehyde complex at 532 nm.

TL;DR: A rapid and sensitive method was developed for the quantitative determination of alpha-tocopherol in tissues and plasma of rats and mice and was found to be more complete in acetone than in other solvent systems analyzed.

TL;DR: Data in this report show that the contaminating proteins are skin proteins, especially keratins ranging from 54 to 57 kDa and 65 to 68 kDa.

TL;DR: The rate of elution of polypeptides from a 15% sodium dodecyl sulfate-polyacrylamide gel is dependent on the molecular weight of the individual polypepton, which is in agreement with the results of W. Burnette (Anal.

TL;DR: These assays facilitate the rapid evaluation and direct comparison of epoxide-metabolizing systems in cell homogenates used in short-term mutagenicity assays, cell or organ culture, and possibly in vivo.

TL;DR: The method requires less manipulation than other procedures; it is extremely rapid, sensitive, and inexpensive; these attributes make this procedure a valuable alternative or supplement to the commonly used methods for visualization by hybridization of nucleic acids separated on agarose gels.

TL;DR: Autoradiograms or fluorograms can be quantified by eluting the silver grains from the developed film with 1 M NaOH and measuring the absorbance of the eluate in a conventional spectrometer.