Mechanisms of siderophore iron transport in enteric bacteria.
J Leong,J B Neilands +1 more
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Uptake of 55Fe- and 3H-labeled ferrichrome and kinetically inert lambda-cis-chromic [3H]deferriferrichrome by the S. typhimurium LT-2 enb7 mutant, which is defective in the production of its native siderophore, enterobactin, appears to occur by two concurrent mechanisms.Abstract:
Uptake of 55Fe- and 3H-labeled siderophores and their chronic analogues have been studied in Salmonella typhimurium LT-2 and Escherichia coli K-12. In S. typhimurium LT-2, at least two different mechanisms for siderophore iron transport may be operative. Uptake of 55Fe- and 3H-labeled ferrichrome and kinetically inert lambda-cis-chromic [3H]deferriferrichrome by the S. typhimurium LT-2 enb7 mutant, which is defective in the production of its native siderophore, enterobactin, appears to occur by two concurrent mechanisms. The first mechanism is postulated to involve either rapid uptake of iron released from the ferric complex by cellular reduction without penetration of the complex or ligand or dissociation of the complex and simultaneous uptake of both ligand and iron coupled with simultaneous expulsion of the ligand. The second mechanism appears to consist of slower uptake of the intact ferric complex.read more
Citations
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Journal Article
Complexation of iron by siderophores. A review of their solution and structural chemistry and biological function
Book ChapterDOI
Siderophore mediated absorption of iron
TL;DR: A brief presentation of iron chemistry with emphasis on those aspects relevant to siderophore biochemistry is made in this article, where the underlying chemistry associated with the movement of iron(III) complexes across membranes and the removal of iron from such complexes is discussed in detail.
Journal ArticleDOI
Binding of metals to cell envelopes of Escherichia coli K-12.
TL;DR: Metal-binding capacity data suggest that most metal deposition occurred at the polar head group regions of the constituent membranes or along the peptidoglycan layer, and membrane integrity was not disturbed under these ionic conditions.
Journal ArticleDOI
Coordination Chemistry of Microbial Iron Transport.
TL;DR: A new chapter in siderophore science has emerged with the characterization of what are now called siderocalins, where these proteins bind both ferric and apo-siderophores to inactivate the sIDERophore transport system and hence deny iron to an invading pathogenic microbe.
Journal ArticleDOI
Accumulation of iron by yersiniae.
R D Perry,R R Brubaker +1 more
TL;DR: It is indicated that hemin can serve as a sole source of iron for yersiniae and that the organisms possess an efficient cell-bound transport system for Fe3+.
References
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Journal ArticleDOI
Studies on the chemical nature of clostridial ferredoxin.
Journal ArticleDOI
Iron Uptake in Salmonella typhimurium: Utilization of Exogenous Siderochromes as Iron Carriers
TL;DR: Genetic and physiological data indicate that S. typhimurium has several transport systems of high specificity for a variety of siderochromes produced by other microorganisms.
Journal ArticleDOI
Iron Transport in Salmonella typhimurium: Mutants Blocked in the Biosynthesis of Enterobactin
TL;DR: Transduction experiments showed that the genes for enterobactin biosynthesis are closely linked on the S. typhimurium chromosome, and it is suggested that they form an operon which is repressed by the presence of iron.
Journal ArticleDOI
Enterochelin System of Iron Transport in Escherichia coli: Mutations Affecting Ferric-Enterochelin Esterase
TL;DR: The theory that iron, taken up by the cell as ferric-enterochelin is only available for general cell metabolism after hydrolysis of the ligand by enterochelin esterase is supported.
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