Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin.
TLDR
The results suggest that messenger RNA and nuclear heterogeneous RNA are synthesized separately, and that the transcription of messenger RNA is inhibited by the drug.Abstract:
Cordycepin (3′-deoxyadenosine) suppresses the labeling of messenger RNA in HeLa cells. The drug has no effect on either the labeling of nuclear heterogeneous RNA or on the transport of messenger RNA into the cytoplasm. The results suggest that messenger RNA and nuclear heterogeneous RNA are synthesized separately, and that the transcription of messenger RNA is inhibited by the drug.read more
Citations
More filters
Journal ArticleDOI
Polyadenylic Acid Sequences: Role in Conversion of Nuclear RNA into Messenger RNA
TL;DR: Polyadenylic acid segments containing 150 to 250 nucleotides appear to be covalently linked to heterogeneous nuclear RNA (HnRNA) and messenger RNA (mRNA) in eucaryotic cells.
Journal Article
Induction of the Polyamine-biosynthetic Enzymes in Mouse Epidermis by Tumor-promoting Agents
TL;DR: The stimulation of both enzyme activities was dependent on the dose of TPA applied and correlated well with the promoting ability of these doses on mouse skin, and enzyme half-lives of 17 and 41 min were obtained for ornithine and S-adenosyl-L-methionine decarboxylase, respectively.
Journal ArticleDOI
A Polynucleotide Segment Rich in Adenylic Acid in the Rapidly-Labeled Polyribosomal RNA Component of Mouse Sarcoma 180 Ascites Cells
TL;DR: The rapidly-labeled polyribosomal RNA component from mouse sarcoma 180 cells is retained on nitrocellulose (Millipore) membrane-filters at high ionic strength due to the presence of a polynucleotide sequence rich in adenylic acid that resists both T(1) and pancreatic RNase digestion.
Journal ArticleDOI
Organization, transcription, and regulation in the animal genome.
Eric H. Davidson,Roy J. Britten +1 more
TL;DR: Recent experimental information in areas of animal cell molecular biology which are relevant to the mechanism of gene regulation are concerned, with particular reference to the frequency of structural gene sequences, mRNA turnover, and the interpretation of dipteran complementation groups.
Journal ArticleDOI
Biogenesis of mRNA: Genetic Regulation in Mammalian Cells
TL;DR: The experimental description of the mechanics as well as the regulation of bacterial protein synthesis appears to have entered a final stage and the challenge of understanding protein synithesis in mammalian cells in detail comparable to the understanding in bacteria is presented.
References
More filters
Journal ArticleDOI
Amino Acid Metabolism in Mammalian Cell Cultures
TL;DR: The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in either monolayer or suspension.
Journal ArticleDOI
Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells
TL;DR: Three heterogeneous RNA species in HeLa cells have been characterized with respect to kinetics of labeling, turnover and location, showing that the nucleoplasmic, cytoplasmic and polyribosome-associated messenger fractions are relatively unaffected by doses of actinomycin which completely inhibit ribosomal RNA synthesis.
Journal ArticleDOI
Messenger RNA-protein complexes and newly synthesized ribosomal subunits: analysis of free particles and components of polyribosomes.
Robert P. Perry,Dawn E. Kelley +1 more
TL;DR: The present experiments indicate that this extra protein, termed accessory protein, is released from the subunits when they are incorporated into polyribosomes as a polydisperse array of ribonucleoprotein complexes with a relatively uniform RNA to protein ratio.
Journal ArticleDOI
Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes.
TL;DR: base composition and kinetics data strongly suggest that no relationship of precursor-to-product type or, at any rate, not a simple one, exists between the heavy non-ribosomal type nuclear RNA and the cytoplasmic messenger RNA.
Journal ArticleDOI
Rapidly labeled HeLa cell nuclear RNA: I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA
TL;DR: The rapidly labeled RNA from HeLa cells has been further studied in this article, showing that 50% of the [3H]uridine incorporated into RNA in four to five minutes is found in this heterogeneous fraction.