Book ChapterDOI
Methods for Observing and Quantifying Muscle Satellite Cell Motility and Invasion In Vitro.
Dane K. Lund,Dane K. Lund,Patrick McAnulty,Patrick McAnulty,Ashley L. Siegel,D.D.W. Cornelison +5 more
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TLDR
Three protocols developed in the group for quantitatively analyzing satellite cell motility over time are described, which allow identification and longitudinal evaluation of individual cells over time and quantification of variations in motility due to intrinsic or extrinsic factors.Abstract:
Motility and/or chemotaxis of satellite cells has been suggested or observed in multiple in vitro and in vivo contexts. Satellite cell motility also affects the efficiency of muscle regeneration, particularly in the context of engrafted exogenous cells. Consequently, there is keen interest in determining what cell-autonomous and environmental factors influence satellite cell motility and chemotaxis in vitro and in vivo. In addition, the ability of activated satellite cells to relocate in vivo would suggest that they must be able to invade and transit through the extracellular matrix (ECM), which is supported by studies in which alteration or addition of matrix metalloprotease (MMP) activity enhanced the spread of engrafted satellite cells. However, despite its potential importance, analysis of satellite cell motility or invasion quantitatively even in an in vitro setting can be difficult; one of the most powerful techniques for overcoming these difficulties is timelapse microscopy. Identification and longitudinal evaluation of individual cells over time permits not only quantification of variations in motility due to intrinsic or extrinsic factors, it permits observation and analysis of other (frequently unsuspected) cellular activities as well. We describe here three protocols developed in our group for quantitatively analyzing satellite cell motility over time in two dimensions on purified ECM substrates, in three dimensions on a living myofiber, and in three dimensions through an artificial matrix.read more
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Book ChapterDOI
Muscle Stem Cells: A Model System for Adult Stem Cell Biology.
TL;DR: Background in the field is provided and recent advances in the understanding of muscle stem cell function and dysfunction are discussed, particularly in the case of aging, and the potential involvement of Muscle stem cells in genetic diseases such as the muscular dystrophies are discussed.
References
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Journal ArticleDOI
Neural cell adhesion molecule (NCAM) marks adult myogenic cells committed to differentiation.
TL;DR: Investigation of neural cell adhesion molecule (NCAM), a multifunctional cell-surface protein that has previously been associated with muscle regeneration, was significant and revealed that not only is NCAM localized to discrete areas of the plasma membrane, it is also a very early marker of commitment to terminal differentiation.
Journal ArticleDOI
Growth factor stimulation of matrix metalloproteinase expression and myoblast migration and invasion in vitro
TL;DR: Observations support the hypothesis that MMP activity is a necessary component of growth factor-mediated myoblast migration but suggest that other consequences of growth factors signaling are also necessary for migration to occur.
Journal ArticleDOI
Muscle precursor cells invade and repopulate freeze-killed muscles.
TL;DR: It is shown, in the mouse, that an extensor digitorum longus (EDL) muscle, killed by repeated freezing and thawing, repopulated with mpc and grafted into a nu/nu or tolerant AKR host mouse, is capable of supporting muscle formation.
Journal ArticleDOI
Activation and localization of matrix metalloproteinase-2 and -9 in the skeletal muscle of the muscular dystrophy dog (CXMDJ)
Kazuhiro Fukushima,Akinori Nakamura,Hideho Ueda,Katsutoshi Yuasa,Kunihiro Yoshida,Shin'ichi Takeda,Shu-ichi Ikeda +6 more
TL;DR: MMP-2 and MMP-9 are likely involved in the pathology of dystrophin-deficient skeletal muscle and may be involved predominantly in the inflammatory process during muscle degeneration.
Journal ArticleDOI
Sdf-1 (CXCL12) improves skeletal muscle regeneration via the mobilisation of Cxcr4 and CD34 expressing cells
Edyta Brzoska,Magdalena Kowalewska,Agnieszka Markowska-Zagrajek,Kamil Kowalski,Karolina Archacka,Malgorzata Zimowska,Iwona Grabowska,Areta M. Czerwinska,Magdalena Czarnecka-Góra,Wladyslawa Streminska,Katarzyna Jańczyk-Ilach,Maria A. Ciemerych +11 more
TL;DR: The regeneration of skeletal muscles involves satellite cells, which are muscle‐specific precursor cells that follow a myogenic program, and participation of these cells in the repair process depends on their precise mobilisation to the site of the injury.