Journal ArticleDOI
Methods for the determination of binding constants by capillary electrophoresis
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TLDR
Six different CE‐based techniques for evaluating binding constants are reviewed, and examples of each method, and recent references on its use are given.Abstract:
Apparent equilibrium constants for molecular association (e.g., association constants, binding constants, dissociation constants, partition coefficients) can be determined with a variety of different capillary electrophoresis (CE) approaches. In many cases, the investigated association behavior is between a smaller molecule or ion (i.e., the solute, drug, or analyte of interest) and a larger entity (e.g., proteins, micelles, polymers, chiral selectors such as cyclodextrins, etc.). Each experimental approach has advantages and disadvantages. Frequently, it is the nature of the system being evaluated that determines the optimal experimental approach. Six different CE-based techniques for evaluating binding constants are reviewed. Examples of each method, and recent references on its use are given.read more
Citations
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Drug-protein binding: a critical review of analytical tools.
TL;DR: This review focuses on the most important approaches used to characterize drug–protein binding and the binding affinity ranges, information accessibility, material consumption, and throughput are compared for each method.
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Enantioseparations in capillary electromigration techniques: recent developments and future trends
TL;DR: This review summarizes the current status of enantioseparations using capillary electromigration techniques and gives the authors insights on the selected fundamental aspects and future trends in this field.
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Overview of capillary electrophoresis and capillary electrochromatography.
TL;DR: An overview on the current status of capillary electrophoresis (CE) and capillary electrochromatography (CEC) and the application, advantages and limitations of CEC are covered.
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Determination of dissociation constants of flavonoids by capillary electrophoresis.
TL;DR: Ionization constants of some flavanols and flavonols are determined by capillary zone electrophoresis (CZE) and the pKa values obtained are compared with those calculated by the SPARC computational program.
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Biomolecular interactions and tools for their recognition: focus on the quartz crystal microbalance and its diverse surface chemistries and applications.
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References
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The Determination of Enzyme Dissociation Constants
Hans Lineweaver,Dean Burk +1 more
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A Spectrophotometric Investigation of the Interaction of Iodine with Aromatic Hydrocarbons
H. A. Benesi,Joel H. Hildebrand +1 more
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Electrokinetic separations with micellar solutions and open-tubular capillaries
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Binding Constants: The Measurement of Molecular Complex Stability
TL;DR: This paper presents a method for quantifying theSolubility of Binding Constants using Optical Absorption Spectroscopy and Magnetic Resonance spectroscopy, and its applications in medicine and materials science.
Journal ArticleDOI
Electrokinetic chromatography with micellar solution and open-tubular capillary
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