Journal ArticleDOI
Micropropagation of Cereus peruvianus mill. (cactaceae) by areole activation
Reads0
Chats0
TLDR
The frequency of shoot formation in the BA or kinetin and NAA or IAA combinations indicated that any of the combinations tested can be used for multiplication ofC.Abstract:
Currently,Cereus peruvianus plants can be rapidly clonedin vitro via adventitious organogenesis using callus cultures; however, somaclonal variation is a problem. A method is described herein using lateral bud explants to produce multiple shoots for clonal propagation. Apical and lateral explants were cultured on MS (Murashige and Skoog, 1962) media with factorial combinations of the auxins indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and cytokinins 6-ben-zyladenine (BA) and N-(2-furanyl-methyl)-1-purine-6 amine (kinetin) at the concentrations 0.0, 0.01, 0.1, 1.0 mg“l−1. Positive results were obtained from the lateral explants in all conditions tested, but apical explants did not respond toin vitro multiplication ofC. peruvianus cactus at all growth regulator combinations tested. Formation of axillary shoots inC. peruvianus seems most frequent in medium containing BA at 1.0 mg·l−1 (4.44 μM) and IAA or NAA at 1.0 mg·l−1 (5.71 μM or 5.37 μM respectively), but the frequency of shoot formation in the BA or kinetin and NAA or IAA combinations indicated that any of the combinations tested can be used for multiplication ofC. peruvianus plants regenerated from callus tissue culture. Root formation occurred in all (100%) of the cactus shoots after 9 wk in the same culture medium. All the cacti that developed at the different auxin and cytokin combinations continued growth after transfer to a potting mix of red earth (Paleudult) and ground river sand (1∶1).read more
Citations
More filters
Journal ArticleDOI
Opuntia and Other Cacti: Applications and Biotechnological Insights
TL;DR: The present review outlines some of the older and more recent research on the properties and applications for Opuntia and other cacti especially as they might apply towards agricultural sustainability.
Journal ArticleDOI
Micropropagation of 21 species of Mexican cacti by axillary proliferation
Eugenio Pérez Molphe Balch,Martha Evelia Pérez Reyes,Enrique Villalobos Amador,Ernestina Meza Rangel,Leticia del Rocío Morones Ruiz,Hugo Jesús Lizalde Viramontes +5 more
TL;DR: The requirements of growth regulators for optimal shoot proliferation, the velocity of the response, and the number of buds produced by explant were different among the genera and species studied.
Journal ArticleDOI
Micropropagation of Cacti—a Review
J. Lema-Ruminska,Dariusz Kulus +1 more
TL;DR: The achievements, current state and future prospects of cacti micropropagation methods are discussed, and an overview of cactus multiplication by areole activation, direct and indirect organogenesis (caulogenesis and rhizogenesis) and somatic embryogenesis is provided, as well as consider acclimatization.
Journal ArticleDOI
Tissue culture of ornamental cacti
Eugenio Pérez-Molphe-Balch,María del Socorro Santos-Díaz,Rafael Ramírez-Malagón,Neftalí Ochoa-Alejo +3 more
TL;DR: The state-of-the-art of tissue culture techniques used for ornamental cacti are presented and selected suggestions for solving a number of the problems faced by members of the Cactaceae family are selected.
Journal ArticleDOI
Plant regeneration of three Opuntia genotypes used as human food
Pedro A. Garcia-Saucedo,Maribel Valdez-Morales,María Elena Valverde,Andrés Cruz-Hernández,Octavio Paredes-López +4 more
TL;DR: The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Book
Encyclopedia of Chemical Technology
TL;DR: Encyclopedia of Chemical Technology The Third Edition of the Encycled encyclopedia of chemical technology as mentioned in this paper is built on the solid foundation of the previous editions of the encyclopedia, which has been updated and updated and many new subjects have been added to reflect changes in chemical technology through the 1970s.
Journal ArticleDOI
Somaclonal variation — a novel source of variability from cell cultures for plant improvement
Philip J. Larkin,W. R. Scowcroft +1 more
TL;DR: It is argued that this variation in plant cell culture itself generates genetic variability (somaclonal variation) that may be employed to enhance the exchange required in sexual hybrids for the introgression of desirable alien genes into a crop species.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more