Molecular characterisation of grapevine yellows associated phytoplasmas of the stolbur-group based on RFLP-analysis of non-ribosomal DNA
M. Langer,M. Maixner +1 more
Reads0
Chats0
TLDR
A specific association of the three isolates to different alternative host plants is discussed and a previously unknown isolate was detected in grapevine, vectors and the newly identified herbaceous host Calystegia sepium.Abstract:
Grapevines, alternative host plants, and vectors from different viticultural areas of Germany were surveyed for the presence of stolbur-group phytoplasmas that are associated with grapevine yellows. Isolates from these field samples and periwinkle isolates of stolbur were characterised by RFLP-analysis of non-ribosomal DNA fragments using a combination of 6 polymorphic primer-enzyme combinations. Three of the 5 different restriction profiles that could be distinguished were found in grapevine and other field samples of plants and insects. A previously unknown isolate was detected in grapevine, vectors and the newly identified herbaceous host Calystegia sepium . The further analysis of the phylogenetic relationship between the 5 RFLP groups revealed a close relationship between two of the isolates associated with grapevine yellows which were also found in the two Convolvulaceae Convolvulus arvensis and Calystegia sepium . The third isolate which was detected in grapevine and stinging nettle Urtica dioica is more closely related to a periwinkle isolate obtained from Lavandula officinalis than to the other two grapevine yellows isolates. Based on the data of the field survey a specific association of the three isolates to different alternative host plants is discussed.read more
Citations
More filters
Journal ArticleDOI
‘Candidatus Phytoplasma phoenicium’ associated with almond witches’-broom disease: from draft genome to genetic diversity among strain populations
Fabio Quaglino,Michael Kube,M. Jawhari,Yusuf Abou-Jawdah,Christin Siewert,E. Choueiri,H. Sobh,Paola Casati,Rosemarie Tedeschi,Marina Molino Lova,Alberto Alma,Piero Attilio Bianco +11 more
TL;DR: The genetic diversity within ‘Ca. P. phoenicium’ strain populations in Lebanon suggested that AlmWB disease could be associated with phytoplasma strains derived from the adaptation of an original strain to diverse hosts.
Journal ArticleDOI
Phytoplasmas and Phytoplasma Diseases: A Severe Threat to Agriculture
TL;DR: Several economically relevant phy toplasma-associated diseases are described and an update of phytoplasma taxonomy and major biological and molecular features ofphytoplasmas are reported.
Journal ArticleDOI
Phytoplasmas: diversity, taxonomy, and epidemiology.
TL;DR: The possibility to design specific primers for highly conserved genes such as 16S ribosomal gene together with the use of molecular probes randomly cloned from phytoplasma genome, allowed discriminating and molecularly classifying them.
Journal ArticleDOI
'Candidatus Phytoplasma solani', a novel taxon associated with stolbur- and bois noir-related diseases of plants.
Fabio Quaglino,Yan Zhao,Paola Casati,D. Bulgari,Piero Attilio Bianco,Wei Wei,Robert E. Davis +6 more
TL;DR: Based on distinct molecular and biological properties, stolbur- and bois noir-associated strains are proposed to represent a novel species level taxon, 'Ca.
Journal ArticleDOI
Real‐time PCR detection systems for Flavescence dorée and Bois noir phytoplasmas in grapevine: comparison with conventional PCR detection and application in diagnostics
TL;DR: A new real-time PCR detection system was developed for grapevine yellows using TaqMan minor groove binder probes and including two amplicons for group-specific detection of Flavescence doree and Bois noir phy toplasmas, plus a universal phytoplasma amplicon.
References
More filters
Journal ArticleDOI
Confidence limits on phylogenies: an approach using the bootstrap.
TL;DR: The recently‐developed statistical method known as the “bootstrap” can be used to place confidence intervals on phylogenies and shows significant evidence for a group if it is defined by three or more characters.
Journal ArticleDOI
Analysis of population genetic structure with RAPD markers
Michael Lynch,Brook G. Milligan +1 more
TL;DR: Estimators for several population‐genetic parameters (gene and genotype frequencies, within‐ and between‐population heterozygosities, degree of inbreeding and population subdivision, and degree of individual relatedness) are presented along with expressions for their sampling variances.
Journal ArticleDOI
Revised Classification Scheme of Phytoplasmas based on RFLP Analyses of 16S rRNA and Ribosomal Protein Gene Sequences
TL;DR: The phytoplasma 16Sr groups were consistent with the phylogenetic groups (subclades) defined by phylogenetic analysis of near-full-length 16S rRNA gene sequences, indicating that the RFLP-based groups are phylogenetically valid.
Journal ArticleDOI
Data from amplified fragment length polymorphism (AFLP) markers show indication of size homoplasy and of a relationship between degree of homoplasy and fragment size.
TL;DR: Results from population samples of the two plant species show that there is a negative relationship between AFLP fragment size and fragment population frequency, and Monte Carlo simulations reveal that size homoplasy, arising from pulling together nonhomologous fragments of the same size, generates patterns similar to those observed in P. lunatus and L. perenne.
Journal ArticleDOI
Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms
TL;DR: Regions representing about 80% of the 16S rDNA sequences of 40 mycoplasmalike organism (MLO) strains from North America, Asia, and Europe were amplified by polymerase chain reaction (PCR) using a primer pair designed on the basis of an MLO16S rRNA gene.