Journal ArticleDOI
Molecular characterization of a partitivirus from the plant pathogenic ascomycete Rosellinia necatrix
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TLDR
The name Rosellinia necatrix partitivirus 1-W8 (RnPV1- W8) is coined based on viral particle morphology and sequence information based oniral particle morphologyand sequence information.Abstract:
The W8 isolate of the phytopathogenic fungus, Rosellinia necatrix that causes white root rot, contained three segments of double-stranded (ds) RNA, namely L1, L2 and M. Purified viral particles of about 25 nm in diameter contained an RNA segment with almost the same mobility as M-dsRNA, but the band was sensitive to S1 nuclease. Molecular analysis revealed that M-dsRNA consisted of two (RNA 1 and RNA 2) similarly sized species of 2299 and 2279 bp excluding an interrupted poly (A or U) tail of 16-51 bp. The predicted largest open reading frame in RNA 1 and RNA 2 was similar to those of RNA dependent RNA polymerase (RdRp) and coat protein (CP), respectively, encoded by the family Partitiviridae. The non-coding regions (NCR) of the two segments were similar (approximately 70% base identity) at the 5' end, but different at the 3' end. The NCR at the 3' end contained adenosine-uracil rich elements (AREs) in both segments. Northern analyses revealed RNA 1 and RNA 2 in mycelial and viral particle fractions. We coined the name Rosellinia necatrix partitivirus 1-W8 (RnPV1-W8) for M-dsRNA based on viral particle morphology and sequence information.read more
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Journal ArticleDOI
Viruses of plant pathogenic fungi.
Said A. Ghabrial,Nobuhiro Suzuki +1 more
TL;DR: Select mycoviruses that cause debilitating diseases and/or reduce the virulence of their phytopathogenic fungal hosts are discussed and such fungal-virus systems are valuable for the development of novel biocontol strategies and for gaining an insight into the molecular basis of fungal virulence.
Journal ArticleDOI
Mycoviruses of filamentous fungi and their relevance to plant pathology
TL;DR: Two hypotheses for the origin of mycoviruses of plant pathogens are discussed: the first that they are of unknown but ancient origin and have coevolved along with their hosts, and the second that they have relatively recently moved from a fungal plant host into the fungus.
Journal ArticleDOI
A novel bipartite double-stranded RNA Mycovirus from the white root rot Fungus Rosellinia necatrix: molecular and biological characterization, taxonomic considerations, and potential for biological control.
TL;DR: Results indicate that the W779 virus is a novel bipartite dsRNA virus with potential for biological control (virocontrol), named Rosellinia necatrix megabirnavirus 1 (RnMBV1), that possibly belongs to a new virus family.
Journal ArticleDOI
Multiple virus infection in a single strain of Fusarium poae shown by deep sequencing
TL;DR: Many bands were detected on an electrophoretic profile of double-stranded (ds) RNA preparation from a single strain of Fusarium poae isolated from wheat, finding the RNA virome of the strain was found to be highly diverse.
Journal ArticleDOI
Hypovirulence of the Phytopathogenic Fungus Botryosphaeria dothidea: Association with a Coinfecting Chrysovirus and a Partitivirus
TL;DR: BdCV1 appears to be a good candidate for the biological control of the serious disease induced by B. dothidea and of a chrysovirus associated with the hypovirulence of a phytopathogenic fungus.
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Chyi-Ying A. Chen,Ann-Bin Shyu +1 more
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Journal ArticleDOI
Biological Control of Chestnut Blight
TL;DR: If a way can be found to help the spread here of strains of the fungus with controlling agents, it may be possible to save the American chestnut trees in the authors' eastern forests.