Molecular genotyping of methicillin-resistant Staphylococcus aureus via fluorophore-enhanced repetitive-sequence PCR.
TLDR
A rapid DNA fingerprinting method that exploits PCR amplification from a DNA repeat sequence in MRSA is described and two PCR fingerprinting methods which use an oligonucleotide primer based on a repetitive sequence found in Mycoplasma pneumoniae are presented.Abstract:
Methicillin resistance in Staphylococcus aureus is a frequent cause of nosocomial and community-acquired infections. Accurate, rapid epidemiologic typing is crucial to the identification of the source and spread of infectious disease and could provide detailed information on the generation of methicillin-resistant S. aureus (MRSA) strains. The high degree of genetic relatedness of MRSA strains has precluded the use of more conventional methods of genetic fingerprinting. A rapid DNA fingerprinting method that exploits PCR amplification from a DNA repeat sequence in MRSA is described. The random chromosomal distribution of this repeat sequence provides an ideal target for detecting DNA fragment patterns specific to individual MRSA strains. Two PCR fingerprinting methods which use an oligonucleotide primer based on a repetitive sequence found in Mycoplasma pneumoniae are presented. The repetitive element sequence-based PCR (rep-PCR) and fluorophore-enhanced rep-PCR (FERP) can identify epidemic strains among background MRSA. The combination of oligonucleotide primers labeled with different fluorescent dyes allowed simultaneous FERP fingerprinting and mecA gene detection. Eight different fingerprint patterns were observed in MRSA strains collected from different sources. These techniques provide a rapid discriminatory means of molecular epidemiologic typing of MRSA involved in nosocomial infections.read more
Citations
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Principles and Applications of Methods for DNA-Based Typing of Microbial Organisms
D. Michael Olive,Pamela Bean +1 more
TL;DR: Outbreaks of infectious disease often result from exposure to a common source of the etiologic agent, which is derived from a single cell whose progeny are genetically identical or closely related to the source organism.
Journal ArticleDOI
Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology
Sahreena Lakhundi,Kunyan Zhang +1 more
TL;DR: The origin of MRSA is described, with emphasis on the diverse nature of staphylococcal cassette chromosome mec (SCCmec).
Journal ArticleDOI
Community-acquired, methicillin-resistant and methicillin-susceptible Staphylococcus aureus musculoskeletal infections in children.
Gerardo Martínez-Aguilar,Ana Avalos-Mishaan,Kristina G. Hulten,Wendy A. Hammerman,Edward O. Mason,Sheldon L. Kaplan +5 more
TL;DR: Febrile days and hospital days were greater in children with musculoskeletal infection caused by MRSA than in those affected by MSSA, but no significant differences were found in the final outcome.
Journal ArticleDOI
Prospective comparison of risk factors and demographic and clinical characteristics of community-acquired, methicillin-resistant versus methicillin-susceptible Staphylococcus aureus infection in children.
TL;DR: The presence of risk factors associated with methicillin resistance among patients, and their household contacts, with CA-MRSA and CA-MSSA and MRSA is an established, community-acquired pathogen in this area.
Journal ArticleDOI
Application of Molecular Techniques to the Study of Hospital Infection
Aparajita Singh,Richard V. Goering,Shabbir Simjee,Steven L. Foley,Marcus J. Zervos,Marcus J. Zervos +5 more
TL;DR: The integration of molecular typing with conventional hospital epidemiologic surveillance has been proven to be cost-effective due to the associated reduction in the number of nosocomial infections.
References
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Journal ArticleDOI
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Journal Article
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