New Classification of Neisseria meningitidis by Means of Bactericidal Reactions
Ronald Gold,Frederic A. Wyle +1 more
TLDR
A bactericidal assay is described which allows identification of distinct serotypes within a serogroup of Neisseria meningitidis, and six different serotypes, containing one or two factors, were identified among 16 group C strains examined.Abstract:Â
A bactericidal assay is described which allows identification of distinct serotypes within a serogroup of Neisseria meningitidis. Antisera produced in rabbits against seven group C strains by two intravenous inoculations of live organisms were found to contain two types of bactericidal antibodies. One, directed against the group-specific polysaccharide, caused various degrees of killing of all strains. Absorption of this antibody by purified group C polysaccharide revealed the presence of the second bactericidal antibody. This antibody was directed against antigenically distinct factors associated with serotype specificity. Extensive cross-absorption yielded antisera with activity directed against four separate factors. The presence of a factor in a strain was indicated by its susceptibility to killing by antisera containing antibody to that factor. A serotype was defined by the particular combination of factors. Six different serotypes, containing one or two factors, were identified among 16 group C strains examined.read more
Citations
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Genetic relationships and clonal population structure of serotype 2 strains of Neisseria meningitidis.
Dominique A. Caugant,Wendell D. Zollinger,L F Mocca,C. E. Frasch,Thomas S. Whittam,L O Frøholm,Robert K. Selander +6 more
TL;DR: Recent temporal changes in the frequencies of recovery of pathogenic strains of serotypes 2a and 2b in South Africa and North America resulted from clone replacement in these populations rather than evolutionary modification of the serotype protein of the initially dominant clones.
Journal ArticleDOI
Antibody-dependent cell-mediated antibacterial activity of human mononuclear cells. I. K lymphocytes and monocytes are effective against meningococi in cooperation with human imune sera.
TL;DR: The data suggest the K cells (as well as monocytes) may play a role in host immune defense against pathogenic bacteria and the ADC antibacterial system and previously described ADCC assays are discussed.
Journal ArticleDOI
Human antibody response to three meningococcal outer membrane antigens: comparison by specific hemagglutination assays.
TL;DR: All three antigens may play a role in the broad human immunity following natural infection, according to the results of a purified strain of Neisseria meningitidis.
Journal ArticleDOI
Bactericidal Antibody in Genital Infection Due to Neisseria gonorrhoeae
TL;DR: Bactericidal antibody was not detected in 95% of the specimens of acute-phase serum obtained from women with gonococcal pelvic inflammatory disease, and the convalescent-phase sera of 70% of women with clinically severe pelvicinflammatory disease showed a rise in titer of bactericidal antibody to the infecting strain of N. gonorrhoeae.
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Chemical Analysis of Major Outer Membrane Proteins of Neisseria meningitidis: Comparison of Serotypes 2 and 11
Chao-Ming Tsai,Carl E. Frasch +1 more
TL;DR: Most of the 15 protein serotypes found in group B Neisseria meningitidis have distinct major outer membrane protein patterns when examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) by the Weber-Osborn system.
References
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TL;DR: Results of the present study suggest that natural immunity to meningococcal disease is initiated, reinforced, and broadened by intermittent carriage of different strains ofMeningococci throughout life.
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TL;DR: This work highlights the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds.
Journal Article
Quantitative Studies on the Bactericidal Actions of Serum and Complement: I. A Rapid Photometric Growth Assay for Bactericidal Activity
TL;DR: A rapid photometric assay method is presented for the titration of the bactericidal activity of antisera or of complement, which requires an initial reaction period during which the organisms, antibody, and complement interact.