New Classification of Neisseria meningitidis by Means of Bactericidal Reactions
Ronald Gold,Frederic A. Wyle +1 more
TLDR
A bactericidal assay is described which allows identification of distinct serotypes within a serogroup of Neisseria meningitidis, and six different serotypes, containing one or two factors, were identified among 16 group C strains examined.Abstract:Â
A bactericidal assay is described which allows identification of distinct serotypes within a serogroup of Neisseria meningitidis. Antisera produced in rabbits against seven group C strains by two intravenous inoculations of live organisms were found to contain two types of bactericidal antibodies. One, directed against the group-specific polysaccharide, caused various degrees of killing of all strains. Absorption of this antibody by purified group C polysaccharide revealed the presence of the second bactericidal antibody. This antibody was directed against antigenically distinct factors associated with serotype specificity. Extensive cross-absorption yielded antisera with activity directed against four separate factors. The presence of a factor in a strain was indicated by its susceptibility to killing by antisera containing antibody to that factor. A serotype was defined by the particular combination of factors. Six different serotypes, containing one or two factors, were identified among 16 group C strains examined.read more
Citations
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Protection against group B meningococcal disease. I. Comparison of group-specific and type-specific protection in the chick embryo model.
TL;DR: It is found that protection against group B infection was primarily group specific and type-specific, and the serotype antibody was bactericidal but only slightly protective in the chick embryo where protection is primarily phagocytic in nature.
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Development and characterization of an anti-idiotype antibody to the capsular polysaccharide of Neisseria meningitidis serogroup C.
TL;DR: It is indicated that monoclonal 6F9 contained a surrogate image on the combining antibody site which mimicked meningococcal C polysaccharide, capable of evoking antibodies to the meningitis C poly Saccharide in syngenic and xenogenic species.
Journal ArticleDOI
Problems in the definition of meningococcal serotypes
TL;DR: The use of purified meningococcal capsular polysaccharides as vaccines has proven to be effective in combating epidemics and in the control of endemic diseases.
Journal ArticleDOI
Role of Protein Serotype Antigens in Protection against Disease Due to Neisseria meningitidis
TL;DR: Children under two years of age who have recovered from disease due to groups B and CN and of serotype 2 make antibody to serotypes 2; this fact indicates the potential immunogenicity of the serotype antigen in young children.
Journal ArticleDOI
Identification of an Epidemic Strain of Group C Neisseria meningitidis by Bactericidal Serotyping
TL;DR: The predominance of serotype 2 strains during epidemics at Fort Dix, New Jersey and Fort Lewis, Washington and the increasing prevalence of serotypes 2 in cases both at other recruit training centers and among civilians, strongly suggest the existence of an epidemic strain of Group C meningococcus.
References
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TL;DR: Results of the present study suggest that natural immunity to meningococcal disease is initiated, reinforced, and broadened by intermittent carriage of different strains ofMeningococci throughout life.
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Journal ArticleDOI
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Quantitative Studies on the Bactericidal Actions of Serum and Complement: I. A Rapid Photometric Growth Assay for Bactericidal Activity
TL;DR: A rapid photometric assay method is presented for the titration of the bactericidal activity of antisera or of complement, which requires an initial reaction period during which the organisms, antibody, and complement interact.