New Classification of Neisseria meningitidis by Means of Bactericidal Reactions
Ronald Gold,Frederic A. Wyle +1 more
TLDR
A bactericidal assay is described which allows identification of distinct serotypes within a serogroup of Neisseria meningitidis, and six different serotypes, containing one or two factors, were identified among 16 group C strains examined.Abstract:
A bactericidal assay is described which allows identification of distinct serotypes within a serogroup of Neisseria meningitidis. Antisera produced in rabbits against seven group C strains by two intravenous inoculations of live organisms were found to contain two types of bactericidal antibodies. One, directed against the group-specific polysaccharide, caused various degrees of killing of all strains. Absorption of this antibody by purified group C polysaccharide revealed the presence of the second bactericidal antibody. This antibody was directed against antigenically distinct factors associated with serotype specificity. Extensive cross-absorption yielded antisera with activity directed against four separate factors. The presence of a factor in a strain was indicated by its susceptibility to killing by antisera containing antibody to that factor. A serotype was defined by the particular combination of factors. Six different serotypes, containing one or two factors, were identified among 16 group C strains examined.read more
Citations
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Elaboration of Both the Group W135 and Group Y Capsular Polysaccharides by a Single Strain of Neisseria meningitidis
TL;DR: A single strain (8021) of Neisseria meningitidis, isolated from a child with disseminated meningococcal disease, was found to elaborate two serogroup-specific capsular polysaccharides-Y and W135.
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Propranolol in uræmic osteodystrophy
Dissertation
The temporal and geographical distribution and diversity of disease-associated Neisseria meningitidis genetic types in Europe
TL;DR: The output of this project will be the largest representative molecular epidemiological study of meningococcal disease in Europe, and insights into the geographic and temporal distribution and structuring of disease-associated clonal complexes and antigens and their associations will be given.
Journal ArticleDOI
A study of the protein-polysaccharide complex of Neisseria meningitidis serogroup A.
TL;DR: The new three-stage technique of isolating and purifying yields a protein-polysaccharide complex homogenous with respect to antigenicity and exhibiting also homogeneity by disc-electrophoresis and ultracentrifugation.
Journal ArticleDOI
Neisseria meningitidis strains isolated in CSR from cerebrospinal fluid: preepidemic shift in serogroup and serotype distribution?
TL;DR: Changes in the distribution of the capsular and noncapsular antigens on N. meningitidis strains from CSR are characteristic of epidemic situation and come in a context of rising meningococcal morbidity and a shift in the morbidity into older age groups.
References
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Journal Article
Quantitative Studies on the Bactericidal Actions of Serum and Complement: I. A Rapid Photometric Growth Assay for Bactericidal Activity
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