Proceedings ArticleDOI
New method of holographic three-dimensional tracking of living cells exploiting their morphological properties
Pasquale Memmolo,Maria Iannone,Maurizio Ventre,Paolo A. Netti,Andrea Finizio,Melania Paturzo,Pietro Ferraro +6 more
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TLDR
In this paper, a new strategy of 3D tracking of living cells by digital holography microscopy based on the morphological changes of cells during the migration is proposed, and a comparison with other 3D-tracking methods is accomplished.Abstract:
We propose a new strategy of three-dimensional (3D) tracking of living cells by digital holography microscopy based on the morphological changes of cells during the migration. A comparison with other 3D tracking methods is accomplished.read more
References
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Journal ArticleDOI
Focus plane detection criteria in digital holography microscopy by amplitude analysis
TL;DR: A focus plane determination method that computes the digital refocus distance of an object investigated by digital holographic microscopy working in transmission and it is shown that when the focus distance is reached, the integrated amplitude is minimum for pure amplitude object and maximum for pure phase object.
Journal ArticleDOI
Automatic focusing in digital holography and its application to stretched holograms
Pasquale Memmolo,Cosimo Distante,Melania Paturzo,Andrea Finizio,Pietro Ferraro,Bahrain Javidi +5 more
TL;DR: An algorithm for automatically estimating the in-focus image and recovering the correct reconstruction distance for speckle holograms and a comparative analysis has been performed with respect to other existing algorithms developed for DH.
Journal ArticleDOI
Automated three-dimensional tracking of living cells by digital holographic microscopy
Patrik Langehanenberg,Lyubomira Ivanova,Ingolf Bernhardt,Steffi Ketelhut,Angelika Vollmer,Dieter Dirksen,Georgi K. Georgiev,Gert von Bally,Björn Kemper +8 more
TL;DR: Results from investigations on sedimenting red blood cells and HT-1080 fibrosarcoma cells in a collagen tissue model demonstrate that DHM enables marker-free automated quantitative dynamic 3-D cell tracking without mechanical focus adjustment.
Journal ArticleDOI
Three-dimensional particle tracking with subnanometer resolution using off-focus images
Zhipeng Zhang,Chia-Hsiang Menq +1 more
TL;DR: A three-dimensional particle tracking algorithm based on microscope off-focus images is presented, enabling prediction of the measurement performance based on calibration data and the validity of the theoretical analysis is also experimentally confirmed.
Journal ArticleDOI
Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization
TL;DR: DHIC allows, from a single recording, to set a posteriori the best conditions for DIC imaging in conjunction with the numerical focusing feature of DH, with the aim to optimize the visualized phase-contrast imaging as favorite representation for bio-scientists.
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