Nonspecific bactericidal activity of the lactoperoxidases-thiocyanate-hydrogen peroxide system of milk against Escherichia coli and some gram-negative pathogens.
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In this paper, the bactericidal activity of the lactoperoxidase-thiocyanate-hydrogen peroxide system in milk and in a synthetic medium was investigated.Abstract:
Two strains of Escherichia coli and one strain each of Salmonella typhimurium and Pseudomonas aeruginosa were killed by the bactericidal activity of the lactoperoxidase-thiocyanate-hydrogen peroxide system in milk and in a synthetic medium. H2O2 was supplied exogenously by glucose oxidase, and glucose was produced at a level which was itself noninhibitory. Two phases were distinguished: the first phase was dependent on the oxidation of SCN(-) by lactoperoxidase and H2O2, which was reversed by reducing agent, and the second phase was dependent on the presence of accumulated H2O2, which was reversed by catalase. The latter enzyme could also reverse the first phase, but only when present in excessive and unphysiological levels. The bactericidal activity was greatest at pH 5 and below, and it depended on the SCN(-)concentration and on the number of organisms. Since raw or heated milk neutralizes the acid barrier against infection in the stomach, the bactericidal system discussed may contribute to the prevention of enteric infections in neonates.read more
Citations
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References
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Journal ArticleDOI
Iodination of bacteria: a bactericidal mechanism
TL;DR: Iodination of the bacteria by the myeloperoxidase-iodide-H2O2 system was demonstrated chemically and radioautographically and suggests the involvement of labile intermediates of iodide oxidation rather than the more stable end products of oxidation such as iodine.
Journal ArticleDOI
Iron-binding Proteins in Milk and Resistance to Escherichia coli Infection in Infants
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Journal ArticleDOI
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J.D. Oram,B. Reiter +1 more
TL;DR: When not fully saturated with iron, bovine, human and goat lactoferrins were bacteriostatic for Bacillus stearothermophilus and Bacillus subtilis in both the presence and absence of trace amounts of metals.
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Influence of Gastric Acidity on Bacterial and Parasitic Enteric Infections: A Perspective
TL;DR: A large number of patients with reduced gastric acid secretion were thought to be especially susceptible to bacterial enteric infections, but the mechanism behind this belief is still unclear.
Journal ArticleDOI
The peroxidase-thiocyanate-hydrogen peroxide antimicrobial system.
TL;DR: The peroxidase-thiocyanate-H 2 O 2 antimicrobial system in inhibited by catalase as well as by a number of low-molecular-weight substance which may be present in the growth media, or in biological fluids is considered.
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