Patent
Oxidatively stable alpha-amylase
Antrim Richard L,Barnett Christopher C,Mitchinson Colin,Scott D. Power,Carol A. Requadt,Solheim Leif P +5 more
TLDR
In this paper, the mutant alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha amylases are disclosed, which have altered oxidative stability and pH performance profiles and/or altered thermal stability as compared to the precursor.Abstract:
Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.read more
Citations
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Journal ArticleDOI
Properties and applications of starch-converting enzymes of the α-amylase family
Marc J. E. C. van der Maarel,Bart A. van der Veen,Joost C.M. Uitdehaag,Hans Leemhuis,Lubbert Dijkhuizen +4 more
TL;DR: The alpha-amylase family of glycosyl hydrolases as discussed by the authors is one of the most common types of enzymes used in industrial applications and has a (beta/alpha) 8-barrel structure with conserved amino acid residues.
Patent
.alpha.-amylase mutants
TL;DR: In this article, a variant of a parent Termamyl-like alpha-amylase has been shown to exhibit an alteration in at least one of the following properties relative to the parent alpha-AMylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH-stability profile, stability towards oxidation, Ca 2+ dependency and specific activity.
Patent
MODIFIED alpha-AMYLASES HAVING ALTERED CALCIUM BINDING PROPERTIES
Richard R. Bott,Andrew Shaw +1 more
TL;DR: In this article, a new calcium binding site is modified by chemically or genetically altering residues associated with that binding site, which has altered performance characteristics, such as low pH starch hydrolysis performance, stability and activity profiles.
Patent
Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
TL;DR: In this article, isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polyptides have been described, which relates to nucleic acid constructs, vectors, and host cells comprising the polyn nucleotides as well as methods of producing and using the poly peptides.
Patent
Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
Helle Outtrup,Lisbeth Hedegaard Hoeck,Bjarne Roenfeldt Nielsen,Torben Vedel Borchert,Vibeke Skovgaard Nielsen,Henrik Bisgard-Frantzen,Allan Svendsen,Carsten Andersen +7 more
TL;DR: In this paper, isolated polypeptides having alpha-amylase activity and isolated nucleic acid sequences encoding the polyptides were described. But the present paper is limited to the case of isolated polyps.
References
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Journal ArticleDOI
Structure and possible catalytic residues of Taka-amylase A
TL;DR: A complete molecular model of Taka-amylase A consisting of 478 amino acid residues was built with the aid of amino acid sequence data and showed a possible productive binding mode between substrate and enzyme.
Patent
Modified enzymes and methods for making same
David A. Estell,James A. Wells +1 more
TL;DR: A cloned subtilisin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme as mentioned in this paper, and the modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.
Patent
MUTANT α-AMYLASE, DETERGENT, DISH WASHING AGENT, AND LIQUEFACTION AGENT
TL;DR: In the mutant α-amylase as discussed by the authors, one or more of the methionine amino acid residues is exchanged with any amino acid residue except for Cys and Met.
Journal ArticleDOI
Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.
TL;DR: The feasibility of improving oxidative stability in proteins by site-directed mutagenesis is demonstrated and Mutant enzymes were expressed in Bacillus subtilis and were found to vary widely in specific activity.
Patent
Bleaching compounds and compositions comprising fatty peroxyacids salts thereof and precursors therefor having amide moieties in the fatty chain
TL;DR: In this paper, a peroxyacid with a polar amide link in the hydrophobic chain was used for surface bleaching of textiles over a wide range of bleach solution temperatures.