Phage conversion in Salmonella enterica serotype Enteritidis: implications for epidemiology.
S. Rankin,D. J. Platt +1 more
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TLDR
This model was used to examine the relationships between phage types within a previously denned clonal lineage, SECLIII, to establish whether or not Enteritidis like Salmonella enterica serotype Typhi and SalmoneLLA serotype Paratyphi B possessed type determining phages.Abstract:
A model system for the study of phage conversion of Salmonella enterica serotype Enteritidis is reported. Temperate phages 1,2,3 and 6 from the phage typing scheme were used to convert several individually recognized phage types into others. Phage type 4 was converted to PT8, PT6a to PT4, PT6a to PT7, PT13 to PT13a and PT15 to PT11; some new phage lysis patterns were also detected. This model was used to examine the relationships between phage types within a previously defined clonal lineage, SECLIII, to establish whether or not Enteritidis like Salmonella enterica serotype Typhi and Salmonella enterica serotype Paratyphi B possessed type determining phages. We were able to convert PT1 to PT20, and PT15 to PT11.read more
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References
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A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
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International increase in Salmonella enteritidis: a new pandemic?
TL;DR: Over the past 5 years Salmonella enteritidis infections in humans have increased on both sides of the Atlantic ocean, and investigations in individual countries suggest it is related to consumption of eggs and poultry which harbour the organism.
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TL;DR: A phage-typing scheme for S. enteritidis is described and to date differentiates 27 types using 10 typing phages.
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Host controlled variation in bacterial viruses
TL;DR: A detailed analysis of two cases of variation in two different bacterial viruses is reported, dealing with nonheritable alterations stemming directly from passage through a new host and not with mutations.