Journal ArticleDOI
Plant regeneration from callus tissue of Gossypium hirsutum L.
TLDR
Development of proembryoids was enhanced by transferring pro Embryoids to media lacking NH4NO3 but containing double the standard KNO3 concentration and gibberellic acid (GA).About:
This article is published in Plant Science Letters.The article was published on 1983-10-01. It has received 164 citations till now. The article focuses on the topics: Kinetin & Callus.read more
Citations
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Genetic engineering of cotton plants and lines
TL;DR: In this article, a method to achieve genetic transformation of cotton plants and lines is described, in which the resulting cotton tissues are subjected to a selection agent or agents to screen for transformants, and the transformed cultures are then induced to commence somatic embryogenesis.
Journal ArticleDOI
Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants.
Ebrahim Firoozabady,David L. DeBoer,Donald J. Merlo,Edward L. Halk,Lorraine N. Amerson,Kay E. Rashka,Elizabeth E. Murray +6 more
TL;DR: Cotton (Gossypium hirsutum L.) cotyledon tissues have been efficiently transformed and plants have been regenerated, facilitating transfer of genes of economic importance to cotton.
Journal ArticleDOI
Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.).
Norma L. Trolinder,Joe R. Goodin +1 more
TL;DR: A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation and a high percentage of infertile plants was observed among the regenerants.
Journal ArticleDOI
Biotechnology Applications of Plant Callus Cultures
TL;DR: This review provides a timely overview of the advancements that have been made with callus cultures in these scientific fields and calls for more callus culture-based products to be developed and market.
Journal ArticleDOI
Induction of Highly Embryogenic Calli and Plant Regeneration in Upland (Gossypium hirsutum L.) and Pima (Gossypium barbadense L.) Cottons
TL;DR: To the authors' knowledge, this is the first report of regeneration of G. barbadense through somatic embryogenesis through somatics embryogenesis of diverse cotton species.
References
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Journal ArticleDOI
Organic Growth Factor Requirements of Tobacco Tissue Cultures
Journal ArticleDOI
Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures
Roy U. Schenk,A. C. Hildebrandt +1 more
TL;DR: The composition of a medium is described that proved useful to culture callus of a variety of monocotyledonous and dicotylingonous plants and facilitated work with single cells and the enzymatic removal of cell walls in related studies.
Journal ArticleDOI
Somatic embryogenesis in suspension cultures of Gossypium klotzschianum anderss
H J Price,R H Smith +1 more
TL;DR: The presence of 2,4-D in the medium resulted in increased vigor of the suspension cultures and subsequently in the formation of many embryoids, but does not seem to be necessary for somatic embryogenesis in cotton.
Journal ArticleDOI
Defined conditions for the initiation and growth of cotton callus in vitro. I. Gossypium arboreum.
TL;DR: The data show that hypocotyl tissue was superior to cotyledon or leaf tissue as the explant source for callus proliferation; the Murashige-Skoog inorganic formulation with 100 myo-inositol, 0.4 thiamine·HCl, 2 indoleacetic acid, 1 kinetin, and 3% glucose solidified by agar was the best medium to initiate callus.
Journal ArticleDOI
Developmental biochemistry of cotton seed embryogenesis and germination: x. Nitrogen flow from arginine to asparagine in germination.
TL;DR: The enzymic basis for the flow of nitrogen from arginine to asparagine during the first 3 days of germination has been measured in extracts from cotton cotyledons and it is shown that the bulk of the nitrogen passed from one generation to the next in dicots is through an asparagin cycle.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
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Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.).
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Genotype specificity of the somatic embryogenesis response in cotton
Norma L. Trolinder,Chen Xhixian +1 more