Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers
Avinash S. Thorat,Nishant A. Sonone,Vrushali V. Choudhari,Rachayya M. Devarumath,K. Harinath Babu +4 more
- Vol. 7, Iss: 1, pp 16-16
TLDR
The results confirmed that somaclonal variation occurs during the process of indirect organogenesis and RAPD and ISSR marker based molecular analysis is a suitable method for an early detection of variation in sugarcane.Abstract:
The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After screening the growth response of callus on six different callus induction media, it was observed that medium no. VI supplemented with 500 mg l−1 of each PVP, Casein hydrolysate and MES buffer showed high amounts of callus in Co 86032 (79.66 ± 0.44%) and Q117 (82.83 ± 1.69%). Addition of PEG 8000 at 2.5% to this medium had a profound impact on inducing somatic embryogenesis in Co 86032 (54.66 ± 1.76%) and Q117 (66.66 ± 2.60%) as compare to control (24.33 ± 1.76%) and (27.33 ± 2.73%), respectively. Cell suspension cultures were established by culturing embryogenic calli in liquid medium showed well established suspension cultures with fever cell aggregates. There was negligible cell division during initial 2 days of incubation and cell count increased rapidly between 2 and 8 days. Further incubation beyond 8 days resulted in a decrease in cell viability. Enhanced callus proliferation in Q117 while enhanced shoot regeneration in Co 86032 was observed from cell suspension culture. The clonal fidelity of in vitro regenerated plants was assessed by using RAPD and ISSR markers. Analysis of the ten RAPD markers indicated that 90.48 and 86.95% true-to-type regenerated plantlets in Co 86032 and Q117, respectively. However, in the ISSR markers, Co 86032 did not show any polymorphism and in the Q117, 92.18% true-to-type plantlets were found. These results confirmed that somaclonal variation occurs during the process of indirect organogenesis and RAPD and ISSR marker based molecular analysis is a suitable method for an early detection of variation in sugarcane.read more
Citations
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A tri-enzyme co-immobilized magnetic complex: Process details, kinetics, thermodynamics and applications.
TL;DR: The tri-enzyme co-immobilized MNPs were successfully utilized for extraction of piperine from black pepper, preparation of sugarcane cell protoplasts, and clarification of papaya juice with prominent yield and reusability vis-à-vis traditional methods.
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Establishment of a somatic embryo regeneration system and expression analysis of somatic embryogenesis-related genes in Chinese chestnut (Castanea mollissima Blume)
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Population genetic structure and diversity analysis in economically important Pandanus odorifer (Forssk.) Kuntze accessions employing ISSR and SSR markers
Noohi Nasim,I. Sriram Sandeep,Ambika Sahoo,Suryasnata Das,Manoj Kumar Panda,Laxmikanta Acharya,V.V. RamaRao,Sanghamitra Nayak,Sujata Mohanty +8 more
TL;DR: The information obtained from genetic diversity analysis could be used for future breeding programmes for Kewda improvement to meet its ever increasing demand in the perfumery industry.
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Enhanced gymnemic acids production in cell suspension cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm. through elicitation
Ganesan Mahendran,Zafar Iqbal,Deepak Kumar,Deepak Kumar,Sanjeet K. Verma,Prasant Kumar Rout,Prasant Kumar Rout,Laiq ur Rahman +7 more
TL;DR: In this article, the authors investigated the effect of sodium nitroprusside (SNP), salicylic acid (SA) and methyl jasmonate (MeJA) on the enhancement of deacylgymnemic acid, gymnemagenin, gymnemic acid IV and gymnemic acids XVII in G. sylvestre cell suspension culture.
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Effect of l-glutamine and casein hydrolysate in the development of somatic embryos from cotyledonary leaf explants in okra (Abelmoschus esculentus L. monech)
Melvin A. Daniel,R. Host Antony David,R. Host Antony David,S. Antony Caesar,M. Ramakrishnan,Veeramuthu Duraipandiyan,Savarimuthu Ignacimuthu,Savarimuthu Ignacimuthu,Naif Abdullah Al-Dhabi +8 more
TL;DR: An efficient protocol for somatic embryogenesis from cotyledonary leaf explants of Okra is developed and no polymorphism was detected revealing the genetic integrity of regenerated plantlets.
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