Journal ArticleDOI
Plant regeneration from protoplast culture of sweet potato (Ipomoea batatas Lam.).
TLDR
Two cultivars of sweet potato plants propagated under in vitro conditions were used as the source of protoplasts and plant regeneration was found to be genotype-dependent, since it was only obtained for cultivar Duclos XI.Abstract:
This is the first report on successful plant regeneration from protoplasts of sweet potato. Two cultivars (Guyana and Duclos XI) of sweet potato plants propagated under in vitro conditions were used as the source of protoplasts. Green compact calli with meristematic areas were induced in the medium supplemented with 2mg1−1 zeatin, and plant regeneration occurred when these calli were transferred onto the medium with zeatin level reduced to 0.25mg1−1. Plant regeneration was found to be genotype-dependent, since it was only obtained for cultivar Duclos XI.read more
Citations
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The origin and evolution of sweet potato (Ipomoea batatas Lam.) and its wild relatives through the cytogenetic approaches.
TL;DR: The distribution and organization of 5S and 18S-5.8S-26S rDNA were studied in 10 varieties of hexaploid Ipomoea batatas, five accessions of tetraploids Ipomeoa trifida, and six related species by using fluorescence in situ hybridization (FISH).
Journal ArticleDOI
Transformation of sweet potato (Ipomoea batatas (L.) Lam.) with Agrobacterium tumefaciens and regeneration of plants expressing cowpea trypsin inhibitor and snowdrop lectin
TL;DR: Transgenic sweet potato plants were obtained following transformation with Agrobacterium tumefaciens LBA4404, and transgenic plants have been obtained that express the β-glucuronidase enzyme, the cowpea trypsin inhibitor and the snowdrop lectin.
Journal ArticleDOI
Plant regeneration from protoplasts: a literature review
S. Roest,L. J. W. Gilissen +1 more
Journal ArticleDOI
Transformation of sweet potato (Ipomoea batatas (L.) Lam.) plants by Agrobacterium rhizogenes
TL;DR: Transgenic sweet potato plants obtained after Agrobacterium rhizogenes -mediated transformation had wrinkled leaves, altered shape of flowers, reduced apical dominance, shortened internodes, small storage roots and abundant, frequently branching roots that showed reduced geotropism.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Nutritional Requirements for Growth of Vicia hajastana Cells and Protoplasts at a Very Low Population Density in Liquid Media
K. N. Kao,M. R. Michayluk +1 more
TL;DR: Vicia cells were able to grow in a mineral-salt solution supplemented with sucrose (or glucose), a few vitamins, and 2,4-dichlorophenoxyacetic acid but were not able to survive when cultured at a low population density unless the medium was supplemented with zeatin, naphthalene-1-acetic Acid, nucleic-acid bases, amino acids, other sugars, sugar alcohols, and organic acids.
Journal ArticleDOI
The isolation, culture and regeneration of Petunia leaf protoplasts.
TL;DR: Protoplasts isolated from varieties of P. hybrida were found to differ in their cultural requirements, and cell colonies derived from individual mesophyll protoplasts grow rapidly upon subculture, to produce callus capable of shoot differentiation and ultimately whole plant formation.
Journal ArticleDOI
Fern callus tissue culture
G. Morel,Ralph H. Wetmore +1 more
TL;DR: It is found that on five of these prothalli the spontaneous appearance of undifferentiated calluses appeared on the upper surface and were formed of a mass of greenish cells containing fewer chloroplastids than those of the remainder of the prothallus.
Journal ArticleDOI
Comparative Studies on Protoplast Regeneration in Herbaceous Species of the Dicotyledoneae Class
TL;DR: Regeneration capacities of isolated protoplasts of 77 dicotyledonous species have been checked and it emerged that the following parameters were appropriate in various taxa: shoot tip regions of axenic shoot cultures, V-KM as protoplast culture medium, high plating densities, repeated dilutions of the regenerated cell clusters, and prolonged culture on V-M agar media.
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