Journal ArticleDOI
Plant regeneration via embryo and shoot bud formation from flower-stalk explants of Oncidium Sweet Sugar
Jen-Tsung Chen,Wei-Chin Chang +1 more
TLDR
Segments taken from flower-stalk internodes of Oncidium Sweet Sugar formed somatic embryos and shoot buds directly from wound surfaces or via nodular masses proliferation within 1.5 months, when cultured on a Gelrite-gelled 1/2-MS basal medium supplemented with thidiazuron in darkness.Abstract:
Segments taken from flower-stalk internodes of Oncidium Sweet Sugar formed somatic embryos and shoot buds directly from wound surfaces or via nodular masses proliferation within 1.5 months, when cultured on a Gelrite-gelled 1/2-MS basal medium supplemented with thidiazuron (0.1–3 mg l−1) in darkness. In light, when subcultured, these nodular masses proliferated into green compact callus, and produced somatic embryos, shoot buds and/or yellowish abnormal structures spontaneously. Supplementing 0.1–1 mg l−1 NAA enhanced embryo formation, but retarded proliferation of shoot buds and yellowish abnormal structures. Somatic embryos that directly formed from wound surfaces of flower stalk explants usually developed into abnormal structures, but the callus-derived embryos could germinate into PLBs and eventually developed to normal plantlets on a hormone-free basal medium for 3–4 weeks. Both the embryo-and shoot bud-derived regenerants developed into healthly plantlets when potted in sphagnum moss and acclimatized in the greenhouse.read more
Citations
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Journal ArticleDOI
Micropropagation of orchids: A review on the potential of different explants
TL;DR: This work endeavours to include the major investigations on explant-based orchid tissue culture starting from the pioneering works of Rotor and Wimber to date to date.
Journal ArticleDOI
Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration
TL;DR: The red light spectrum enhanced induction, proliferation, and the carbohydrate contents of PLBs, as well as subsequent plantlet lengths, while the blue spectrum promoted differentiation, protein accumulation, and enzyme activities in PLBs and developing plantlets.
Journal ArticleDOI
Rapid in vitro propagation of Dendrobium hybrids through direct shoot formation from foliar explants, and protocorm-like bodies
K. P. Martin,Joseph Madassery +1 more
TL;DR: In vitro propagation protocol for Dendrobium hybrids Sonia 17 and 28, two highly priced commercial cut flower cultivars through direct organogenesis from in vitro derived foliar explants was established by subsequent induction of protocorm-like bodies (PLBs) and its conversion to shoots.
Journal ArticleDOI
The Application of Biotechnology to Orchids
TL;DR: An informative and broad overview of orchid biotechnology, addressing several important aspects such as molecular systematics, modern breeding, in vitro morphogenesis, protoplast culture, flowering control, flower color, somaclonal variation, orchid mycorrhiza, pathogen resistance, virus diagnosis and production of virus-free plants.
Journal ArticleDOI
Thidiazuron-induced abnormalities in plant tissue cultures
Yaser Hassan Dewir,Yaser Hassan Dewir,Nurmansyah,Yougasphree Naidoo,Jaime A. Teixeira da Silva +4 more
TL;DR: This review addresses the morphological, physiological, and cytogenetic abnormalities associated with the use of TDZ in vitro, and provides a summary of these abnormalities in several plant species.
References
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Book
Orchids of Asia
Eng Soon Teoh,Tiong Ghee Lim +1 more
TL;DR: Teoh as discussed by the authors described the most important orchid tribes of the Asian lowlands with basic essential information on their cultural needs, hybridization, diseases, and the aesthetic appreciation of these orchids.
Journal ArticleDOI
In vitro flowering of Oncidium varicosum mericlones (orchidaceae)
TL;DR: In vitro flowering of Oncidium varicosum was observed after 8–9 months of culture and it is assumed that the origin of the explants and/or the kind of light and its photoperiod might have triggered the flowering process.
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