Journal ArticleDOI
Plant regeneration via embryo and shoot bud formation from flower-stalk explants of Oncidium Sweet Sugar
Jen-Tsung Chen,Wei-Chin Chang +1 more
TLDR
Segments taken from flower-stalk internodes of Oncidium Sweet Sugar formed somatic embryos and shoot buds directly from wound surfaces or via nodular masses proliferation within 1.5 months, when cultured on a Gelrite-gelled 1/2-MS basal medium supplemented with thidiazuron in darkness.Abstract:
Segments taken from flower-stalk internodes of Oncidium Sweet Sugar formed somatic embryos and shoot buds directly from wound surfaces or via nodular masses proliferation within 1.5 months, when cultured on a Gelrite-gelled 1/2-MS basal medium supplemented with thidiazuron (0.1–3 mg l−1) in darkness. In light, when subcultured, these nodular masses proliferated into green compact callus, and produced somatic embryos, shoot buds and/or yellowish abnormal structures spontaneously. Supplementing 0.1–1 mg l−1 NAA enhanced embryo formation, but retarded proliferation of shoot buds and yellowish abnormal structures. Somatic embryos that directly formed from wound surfaces of flower stalk explants usually developed into abnormal structures, but the callus-derived embryos could germinate into PLBs and eventually developed to normal plantlets on a hormone-free basal medium for 3–4 weeks. Both the embryo-and shoot bud-derived regenerants developed into healthly plantlets when potted in sphagnum moss and acclimatized in the greenhouse.read more
Citations
More filters
Journal ArticleDOI
Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium `Gower Ramsey'
Jen-Tsung Chen,Wei-Chin Chang +1 more
TL;DR: Embryo formation was significantly affected by explant position, and leaf tip segments had a significantly higher embryogenic response than other segments of leaves.
Journal ArticleDOI
Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids
TL;DR: Leaf explants of Paphiopedilum phiIippinense hybrids directly formed adventitious shoots from wound regions within 1 month, when cultured on modified Murashige and Skoog medium free of plant growth regulator in darkness.
Journal ArticleDOI
Abnormalities in somatic embryogenesis caused by 2,4-D: an overview
Claudia Garcia,Alex-Alan Furtado de Almeida,Marcio Gilberto Cardoso Costa,Dahyana Santos Britto,Raul Rene Valle,Stefan Royaert,Jean-Philippe Marelli +6 more
TL;DR: The identification of the main factors that can cause abnormal SE development in different plant species are identified, how SE abnormalities are related to somaclonal variations and which genes may be involved with embryo abnormalities are suggested.
Journal ArticleDOI
In vitro flowering of Bambusa edulis and subsequent plantlet survival
TL;DR: Multiple shoots grown from in vitro, spikelet-derived, somatic embryos of Bambusa edulis were used for in vitro flowering and flowered on Murashige and Skoog medium with 0.5 mM thidiazuron (TDZ) and 30 g l sucrose.
Journal ArticleDOI
Plant regeneration via protocorm-like body formation and shoot multiplication from seed-derived callus of a maudiae type slipper orchid
TL;DR: Tiny seeds from 5-month-old green capsules of a maudiae type slipper orchid, Paphiopedilum Alma Gavaert, were induced to form totipotent callus and regenerated plantlets grew normally when transplanted to containers with sphagnum moss in a shaded greenhouse.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Cytokinin activity of N-phenyl-N′-1, 2,3-thiadiazol-5-ylurea (thidiazuron)
TL;DR: The cytokinin activity of Thidiazuron was similar to that of the highly active N -phenyl- N ′-4-pyridylurea derivatives and to the most active cytokinins of the adenine type.
Journal ArticleDOI
Plant regeneration from callus culture of Cymbidium ensifolium var. misericors.
C. Chang,Wei-Chin Chang +1 more
TL;DR: Totipotent calli of Cymbidium ensifolium var.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey and subsequent plant regeneration.
Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae)
Jen-Tsung Chen,Wei-Chin Chang +1 more