Journal ArticleDOI
Plant regeneration via somatic embryogenesis in cotton
TLDR
An efficient in vitro plant regeneration system characterized by rapid and continuous production of somatic embryos using leaf and stem explants of abnormal seedling as an explant have been developed in Gossypium hirsutum L.Abstract:
An efficient in vitro plant regeneration system characterized by rapid and continuous production of somatic embryos using leaf and stem explants of abnormal seedling as an explant have been developed in Gossypium hirsutum L. Embryogenic callus and somatic embryos have been obtained directly from the explants of cotton abnormal seedlings. Plant growth regulators influenced the induction of cotton somatic embryogenesis. The optimal medium for direct somatic embryogenesis was modified MS medium supplemented with 0.1 mg l-1 ZT and 2 g l-1 activated carbon. On this medium, an average of 28.0 and 28.1 matured somatic embryos formed from per leaf and stem explants respectively. The highest frequency of somatic embryogenesis was 100%. The somatic embryos were converted into normal plantlets when cultured on modified MS medium supplemented with 0.1 mg l-1 ZT. Upon transfer to soil, plants grew well and appeared normal. Plants could be regenerated within 60–80 days. The system of cotton somatic embryogenesis and plant regeneration described here will facilitate the application of plant tissue culture and genetic engineering on cotton genetic improvement.read more
Citations
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Regeneration through somatic embryogenesis of an elite colombian Theobroma cacao L. variety
TL;DR: This is the first report where it is achieved the successful conversion to plantlets (68%) and ex vitro adaptation of a colombian cocoa variety via primary and secondary embryogenesis.
Book ChapterDOI
Plant Genetic Engineering: Problems and Applications
TL;DR: Genetically engineered/modified crops, by Agrobacterium-mediated transformation system are becoming common in many countries and the health concerns on plants, animal and human life due to GM foods is unpredictable.
Journal ArticleDOI
PERKEMBANGAN PENELITIAN INDUKSI KALUS EMBRIOGENIK PADA JARINGAN VEGETATIF TANAMAN KARET KLONAL (Hevea brasiliensis Muell. Arg)
TL;DR: Perbanyakan tanaman karet (Hevea brasiliensis Muell Arg) saat ini masih menggunakan cara konvensional yaitu okulasi yang menjadi faktor pembatas perkembangan kalus.
Efficient plantlet regeneration from nodal explant culture of Blackgram (Vigna mungo L.) Hepper
TL;DR: The synergistic effect of both auxin and cytokinin combination was found to be effective in inducing maximum number of shoots and the plant developed in vitro using nodal cultures were established in pots containing garden soil outside under shade in wound temperature and light conditions.
References
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Journal ArticleDOI
Genetically Transformed Cotton ( Gossypium Hirsutum L.) Plants
TL;DR: Genetically engineered plants of commercial cotton varieties obtained by Agrobacterium–mediated transformation gave rise to transformed calli that were resistant to the antibiotic and expressed the neomycin phosphotransferase enzyme.
Journal ArticleDOI
Transformation of cotton (Gossypium hirsutum L.) via particle bombardment.
TL;DR: Southern hybridization confirmed the presence of the hygromycin resistance gene in embryogenic suspension culture tissue and regenerated plants.
Journal ArticleDOI
Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.).
Norma L. Trolinder,Joe R. Goodin +1 more
TL;DR: A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation and a high percentage of infertile plants was observed among the regenerants.
Journal ArticleDOI
Transformation of Elite Cotton Cultivars via Particle Bombardment of Meristems
TL;DR: Molecular and genetic characterization of primary transformants and their progeny established that foreign genes were stably integrated and transmitted to progeny in a Mendelian fashion.
Journal ArticleDOI
Plant regeneration from callus tissue of Gossypium hirsutum L.
TL;DR: Development of proembryoids was enhanced by transferring pro Embryoids to media lacking NH4NO3 but containing double the standard KNO3 concentration and gibberellic acid (GA).
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