Journal ArticleDOI
Plant regeneration via somatic embryogenesis in cotton
TLDR
An efficient in vitro plant regeneration system characterized by rapid and continuous production of somatic embryos using leaf and stem explants of abnormal seedling as an explant have been developed in Gossypium hirsutum L.Abstract:
An efficient in vitro plant regeneration system characterized by rapid and continuous production of somatic embryos using leaf and stem explants of abnormal seedling as an explant have been developed in Gossypium hirsutum L. Embryogenic callus and somatic embryos have been obtained directly from the explants of cotton abnormal seedlings. Plant growth regulators influenced the induction of cotton somatic embryogenesis. The optimal medium for direct somatic embryogenesis was modified MS medium supplemented with 0.1 mg l-1 ZT and 2 g l-1 activated carbon. On this medium, an average of 28.0 and 28.1 matured somatic embryos formed from per leaf and stem explants respectively. The highest frequency of somatic embryogenesis was 100%. The somatic embryos were converted into normal plantlets when cultured on modified MS medium supplemented with 0.1 mg l-1 ZT. Upon transfer to soil, plants grew well and appeared normal. Plants could be regenerated within 60–80 days. The system of cotton somatic embryogenesis and plant regeneration described here will facilitate the application of plant tissue culture and genetic engineering on cotton genetic improvement.read more
Citations
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Book ChapterDOI
Cell and Tissue Culture Approaches in Relation to Crop Improvement
TL;DR: During the past 25 years, the combined use of recombinant DNA technology, gene transfer methods and cell and tissue culture techniques has led to the efficient transformation and production of transgenics in a wide variety of crop plants.
Journal ArticleDOI
Sensitivity of some quantitative and yield characters of 'Egusi' melon (Colocynthis citrullus L.) to treatment with microtubule inhibitors
TL;DR: An examination of the plants in subsequent generations indicated that all of these traits were stably integrated and expressed in the mutants, tacitly implying that they were fixed and can be introgressed into traditional varieties through backcrossing for further improvement of ‘Egusi’ melon.
Book ChapterDOI
Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures
TL;DR: A detailed procedure to transform cotton embryogenic cell suspension cultures by biolistic bombardment and finds that stable transformation at a high frequency (up to 4% of the transiently expressing cells) is possible.
Journal ArticleDOI
In vitro assay for 2,4-D resistance in transgenic cotton
TL;DR: A rapid method for identifying transgenic plants that has been verified in the field will allow identification of cotton transformants at an early stage of plant development, saving time and improving cultivars containing the 2,4-D resistance trait.
Journal ArticleDOI
Comparison of callus induction and somatic embryogenesis of some Iranian cottons ( Gossypium Spp.) with Coker 312 and histology of somatic embryogenesis
TL;DR: In the histological studies, the different development stages of the embryos (globular, heart, torpedo and cotyledonary) together with callus cells were showed and the optimum medium for the development of somatic embryos was MS medium containing B 5 vitamins, 40 g/l sucrose and without hormone.
References
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Journal ArticleDOI
Genetically Transformed Cotton ( Gossypium Hirsutum L.) Plants
TL;DR: Genetically engineered plants of commercial cotton varieties obtained by Agrobacterium–mediated transformation gave rise to transformed calli that were resistant to the antibiotic and expressed the neomycin phosphotransferase enzyme.
Journal ArticleDOI
Transformation of cotton (Gossypium hirsutum L.) via particle bombardment.
TL;DR: Southern hybridization confirmed the presence of the hygromycin resistance gene in embryogenic suspension culture tissue and regenerated plants.
Journal ArticleDOI
Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.).
Norma L. Trolinder,Joe R. Goodin +1 more
TL;DR: A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation and a high percentage of infertile plants was observed among the regenerants.
Journal ArticleDOI
Transformation of Elite Cotton Cultivars via Particle Bombardment of Meristems
TL;DR: Molecular and genetic characterization of primary transformants and their progeny established that foreign genes were stably integrated and transmitted to progeny in a Mendelian fashion.
Journal ArticleDOI
Plant regeneration from callus tissue of Gossypium hirsutum L.
TL;DR: Development of proembryoids was enhanced by transferring pro Embryoids to media lacking NH4NO3 but containing double the standard KNO3 concentration and gibberellic acid (GA).
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