Journal ArticleDOI
Plant regeneration via somatic embryogenesis in cotton
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TLDR
An efficient in vitro plant regeneration system characterized by rapid and continuous production of somatic embryos using leaf and stem explants of abnormal seedling as an explant have been developed in Gossypium hirsutum L.Abstract:
An efficient in vitro plant regeneration system characterized by rapid and continuous production of somatic embryos using leaf and stem explants of abnormal seedling as an explant have been developed in Gossypium hirsutum L. Embryogenic callus and somatic embryos have been obtained directly from the explants of cotton abnormal seedlings. Plant growth regulators influenced the induction of cotton somatic embryogenesis. The optimal medium for direct somatic embryogenesis was modified MS medium supplemented with 0.1 mg l-1 ZT and 2 g l-1 activated carbon. On this medium, an average of 28.0 and 28.1 matured somatic embryos formed from per leaf and stem explants respectively. The highest frequency of somatic embryogenesis was 100%. The somatic embryos were converted into normal plantlets when cultured on modified MS medium supplemented with 0.1 mg l-1 ZT. Upon transfer to soil, plants grew well and appeared normal. Plants could be regenerated within 60–80 days. The system of cotton somatic embryogenesis and plant regeneration described here will facilitate the application of plant tissue culture and genetic engineering on cotton genetic improvement.read more
Citations
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Respuesta de Zamia incognita L. al cultivo in vitro, una alternativa para su conservación In vitro culture response of Zamia incognita, an alternative for preservation
TL;DR: The regeneration potential of Z incognita plants from leaf explants and zygotic embryos was evaluated and somatic embryo formation in the MB2 medium was achieved without growth regulators or containing 2.4-D alone or in combination with BAP without achieving the conversion process to seedlings.
Journal ArticleDOI
Meristem tip culture in Phragmites australis and genetic fidelity study using SRAP markers
TL;DR: In this article , meristem tip culture was standardized for five clones of Phragmites to evaluate their response and growth, and two clones (IND 89-711 and IND04-1326 ) showed better regeneration and growth.
Journal ArticleDOI
Optimasi perlakuan eksplan pada kultur organ vegetatif tanaman karet (hevea brasiliensis muell. arg.) klon pb 330
Lestari Admojo,Nur Eko Prasetyo +1 more
TL;DR: Hasil penelitian menunjukkan bahwa beberapa optimasi perlakuan sumber eksplan berhasil dilakukan, namun persentasenya sangat rendah akibat ketidakstabilan kalus friabel yang diperoleh, dan adanya hambatan fase diferensiasi selanjutnya.
Posted ContentDOI
Genetic Analysis of callus initiation in Gossypium tomentosum and Gossypium darwinii
TL;DR: The present studies aimed at optimizing the conditions for regeneration of wild species of cotton by obtaining somatic embryogenesis in the G. tomentosum and G. darwinii species to create new germplasm sources.
Journal ArticleDOI
Erratum to: Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.)
Vijaya Naresh Juturu,Gopala Krishna Mekala,Giridara Kumar Surabhi,Pulugurtha Bharadwaja Kirti +3 more
TL;DR: The status of different regeneration and transformation methods in cotton along with the major factors that exert influence in developing cotton transgenics are discussed, besides the chronological progress made in tissue culture and cotton transformation technology.
References
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Journal ArticleDOI
Genetically Transformed Cotton ( Gossypium Hirsutum L.) Plants
TL;DR: Genetically engineered plants of commercial cotton varieties obtained by Agrobacterium–mediated transformation gave rise to transformed calli that were resistant to the antibiotic and expressed the neomycin phosphotransferase enzyme.
Journal ArticleDOI
Transformation of cotton (Gossypium hirsutum L.) via particle bombardment.
TL;DR: Southern hybridization confirmed the presence of the hygromycin resistance gene in embryogenic suspension culture tissue and regenerated plants.
Journal ArticleDOI
Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.).
Norma L. Trolinder,Joe R. Goodin +1 more
TL;DR: A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation and a high percentage of infertile plants was observed among the regenerants.
Journal ArticleDOI
Transformation of Elite Cotton Cultivars via Particle Bombardment of Meristems
TL;DR: Molecular and genetic characterization of primary transformants and their progeny established that foreign genes were stably integrated and transmitted to progeny in a Mendelian fashion.
Journal ArticleDOI
Plant regeneration from callus tissue of Gossypium hirsutum L.
TL;DR: Development of proembryoids was enhanced by transferring pro Embryoids to media lacking NH4NO3 but containing double the standard KNO3 concentration and gibberellic acid (GA).
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