Journal ArticleDOI
Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coli.
Robert L. Perlman,Ira Pastan +1 more
TLDR
It is concluded that cyclic AMP is necessary for the normal utilization of a number of carbon sources by E. coli, because it is required for the synthesis of enzymes involved in their metabolism.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1969-09-24. It has received 190 citations till now. The article focuses on the topics: Cyclase & Galactose.read more
Citations
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Journal ArticleDOI
Cyclic AMP receptor protein: role in transcription activation
TL;DR: The structure of this pleiotropic activator of gene transcription in bacteria and its interaction sites at promoter DNA's as well as the role of this protein in the RNA polymerase-promoter interactions are reviewed.
Journal ArticleDOI
Cyclic Adenosine Monophosphate in Bacteria
Ira Pastan,Robert L. Perlman +1 more
TL;DR: The action of cyclic AMP in E. coli may serve as a model to understand its action on transcriptional and translational processes in eukaryotes.
Journal ArticleDOI
Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic.
Curtiss R rd,Sandra M. Kelly +1 more
TL;DR: Fusaric acid-resistant derivatives with deletions of the Tn10 and adjacent DNA sequences were constructed in S. typhimurium SR-11 strains with or without the virulence plasmid pStSR100, and these delta cya delta crp strains grew more slowly than wild-type strains.
Journal ArticleDOI
Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
TL;DR: It was shown that synthesis of both the plasmid-determined protein colicin E1 and the protein component(s) of the ColE1 relaxation complex is mediated through the c-AMP-catabolite gene activator protein system.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in escherichia coli K12☆
TL;DR: N-methyl-N′-nitro-N-nitrosoguanidine induces at least one mutation per treated cell under conditions permitting over 50 per cent survival and induced auxotrophs are found.
Journal ArticleDOI
Adenosine 3',5'-phosphate in escherichia coli.
Journal ArticleDOI
Regulation of β-Galactosidase Synthesis in Escherichia coli by Cyclic Adenosine 3',5'-Monophosphate
Robert L. Perlman,Ira Pastan +1 more
TL;DR: Cyclic AMP appears to participate in the regulation of β-galactosidase mRNA synthesis at the gene level in Escherichia coli made permeable by treatment with tris(hydroxymethyl)aminomethane and ethylenediamintetraacetic acid.
Journal ArticleDOI
Cyclic 3′5′-AMP: Stimulation of β-galactosidase and tryptophanase induction in E. Coli
Robert L. Perlman,Ira Pastan +1 more
TL;DR: It is reported that cyclic 3′,5′-AMP increases the differential rate of synthesis of two inducible enzymes, β-galactosidase and tryptophanase, when the synthesis of these enzymes is repressed by glucose and pyruvate respectively.