Production and isolation of chitosan from Mucor rouxii.
S A White,P R Farina,I Fulton +2 more
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A method for the lab-scale production and isolation of chitosan (polyglucosamine) from hyphal walls of Mucor rouxii was developed and the last named was the most efficient.Abstract:
A method for the lab-scale production and isolation of chitosan (polyglucosamine) from hyphal walls of Mucor rouxii was developed Hyphal wall yields were generally 16 to 22% on a dry cell weight basis, of which 35 to 40% was glucosamine Chitosan was readily extracted from purified, mycelial walls with acetic, formic, and hydrochloric acids; the last named was the most efficient The yield of chitosan isolated ranged from 4 to 8% of the dry weight of the cell wall materialread more
Citations
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Production, properties, and some new applications of chitin and its derivatives.
TL;DR: Enzymatic procedures for deproteinization of the shells or mold mycelia and for chitin deacetylation were investigated and show that chit in is resistant to enzymatic de acetylation.
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Chitosan based hydrogels and their applications for drug delivery in wound dressings: A review
TL;DR: Applications and structural characteristics of different types of active ingredients, such as growth factors, nanoparticles, nanostructures, and drug loaded chitosan hydrogels are summarized.
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Chitosan-based biodegradable functional films for food packaging applications
TL;DR: Chitosan is the structural material of crustaceans, insects, and fungi, and is the second most abundant biopolymer after cellulose on earth as mentioned in this paper, which can be obtained by deacetylation of chitin.
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Fungal chitosan production and its characterization.
TL;DR: The objective of this investigation was to evaluate the chitosans produced by several species of fungi and found that several of them were similar to each other.
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Physicochemical properties and bioactivity of fungal chitin and chitosan.
TL;DR: Chitinous material extracted from mycelia of Aspergillus niger and Mucor rouxii grown in yeast peptone dextrose broth and tested for antibacterial and eliciting properties significantly reduced lesions caused by Botrytis cinerea and Penicillium expansum in harvested apples.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Isolation, composition, and structure of cell walls of filamentous and yeast-like forms of Mucor rouxii.
TL;DR: Comparative studies were made on their composition and structure to explore possible morphogenetic implications and major quantitative differences were found in protein, purine-pyrimidine, and especially mannose content, all of which were higher in the yeast walls.
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Nutrition, growth, and morphogenesis of mucor rouxii
TL;DR: Variations in temperature and time of incubation, inoculum size, type and concentration of carbon source, type of nitrogen source, and presence of various substances with known action on fungal morphogenesis altered growth in many cases, but did not significantly affect the patterns of vegetative morphogenesis conditioned by each atmosphere of incubators; however, vegetative Morphogenesis was strongly affected by addition of certain chelating agents.
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Relationship of the Major Constituents of the Neurospora crassa Cell Wall to Wild-Type and Colonial Morphology
P. R. Mahadevan,Edward L. Tatum +1 more
TL;DR: The relationship of cell wall to morphology in Neurospora crassa was studied by correlating the levels of structural polymers of the cell wall with wild-type and colonial morphology.
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A mutant of yeast defective in cellular morphogenesis.
Barbara F. Sloat,John R. Pringle +1 more
TL;DR: In the budding yeast Saccharomyces cerevisiae, each bud appears within a ring of chitin formed in the cell wall of the mother cell, but temperature-sensitive mutants defective in gene cdc24 synthesize ch itin at restrictive temperatures, but do not organize it into the discrete rings found in normal cells.