Pyridine Nucleotide-Nitrate Reductase from Extracts of Higher Plants.
Harold J. Evans,Alvin Nason +1 more
TLDR
This article describes the identification of the enzyme nitrate reductase (NR) in the leaves of vascular plants, the first report of NR in plants.Abstract:
This article describes the identification of the enzyme nitrate reductase (NR) in the leaves of vascular plants. Although NR had previously been described in Neurospora, this is the first report of NR in plants. The discovery of NR opened up the field of study of plant nitrogen assimilation and led to the publication of hundreds of articles. NR requires reducing power from NADH, NADPH, or both depending on its location within the plant body and the species. This very complicated enzyme took many years to actually purify. After purification, we learned that it is controlled by phosphorylation/ dephosphorylation. Harold Evans and others later showed NR to be one of the few molybdenumcontaining enzymes in vascular plants. After working on NR, Evans turned his attention to another molybdenum-containing enzyme, dinitrogenase, in soybean (Glycine max) root nodule bacteroids. Harold Evans became President of the American Society of Plant Physiologists and a member of the National Academy of Science based on this discovery and many other contributions.read more
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Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Book ChapterDOI
[24] Nitrate reductase from higher plants
R.H. Hageman,A.J. Reed +1 more
TL;DR: In this paper, the preparation and extraction of nitrate reductase from higher plants is described, where chlorophyllous lamina tissue from illuminated plants well supplied with nitrate is normally used as a source material because of its high activity.
Book ChapterDOI
Nitrate Accumulation in Vegetables
TL;DR: A straightforward relationship between the nitrate concentration of outer leaves of head lettuce and the amounts of fertilizer-nitrite applied within a given planting date and nitrite source is presented in this paper.
Journal ArticleDOI
Hepatic glutathione reductase. I. Purification and general kinetic properties.
Journal ArticleDOI
Nitrate Reductase Activity in Corn Seedlings as Affected by Light and Nitrate Content of Nutrient Media
Richard H. Hageman,Donna Flesher +1 more
TL;DR: It was shown that both light and nitrate are necessary for the formation of nitrate reductase in quantities required by the plant for normal growth.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Absorption of light by chlorophyll solutions
TL;DR: This paper deals with the estimation of chlorophyll in plant extracts by application of absorption coefficients of the isolated solid chlorophylla components, and the question of artifacts is automatically clarified.
Journal ArticleDOI
The extinction coefficients of the reduced band of pyridine nucleotides
B.L. Horecker,Arthur Kornberg +1 more
TL;DR: The quantitative determination of the pyridine nucleotides and of substrates which can be brought into stoichiometric reaction with them has been hampered by the lack of reliable extinction coefficients for these substances.
Journal ArticleDOI
Triphosphopyridine nucleotide-nitrate reductase in Neurospora.
Alvin Nason,Harold J. Evans +1 more
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Nitrate Reductase Activity in Corn Seedlings as Affected by Light and Nitrate Content of Nutrient Media
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