Qualitative and quantitative analysis of rabbit's fat mesenchymal stem cells
Marcelo Paulo Vaccari Mazzetti,Isis Sousa Oliveira,Regiane Miranda-Ferreira,Grasiele Fauaz,Chaibe Nunes Ribeiro,Paulo Oliveira Gomes,Paulo Pontes,Alice T. Ferreira,Lilian Piñero Eça +8 more
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TLDR
The lipectomy of adipose panicule is a very satisfactory method to extract stem cells from fat, quantitatively and qualitatively.Abstract:
PURPOSE: To present an experimental model of qualitative and quantitative analysis of mesenchymal stem cells from fat of rabbits obtained by lipectomy. The fat could be a great source for obtaining mesenchymal stem cells and to create conditions for repairing injured tissues by bioengineering. METHODS: New Zealand rabbits (n= 10) adipose panicle (2-3 cm) were removed by lipectomy, fragmented and washed with PBS and enzymatically dissociated with trypsin/EDTA. Lately, these cells were incubated in culture medium DMEM and after 20 days, was performed quantitative analysis of the accession of first and second mesenchymal cells in cell culture bottles. RESULTS: The fat total cells (CTF) were 1.62 x106 cells/mL and presented 98% of viability. These cells were taken for cultivation and after 20 days were counted 2.88 x106 cells/mL MSC. The same was done and after 20 days we quantified 4.28 x106 cells/mL MSC. CONCLUSION: The lipectomy of adipose panicule is a very satisfactory method to extract stem cells from fat, quantitatively and qualitatively.read more
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Exosomes in mesenchymal stem cells, a new therapeutic strategy for cardiovascular diseases?
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Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance
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A safety study on intrathecal delivery of autologous mesenchymal stromal cells in rabbits directly supporting Phase I human trials.
Bingkun K. Chen,Nathan P. Staff,Andrew M. Knight,Jarred J. Nesbitt,Greg W. Butler,Douglas J. Padley,Joseph E. Parisi,Allan B. Dietz,Anthony J. Windebank +8 more
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Adipose mesenchymal stem cells associated with xenograft in a guided bone regeneration model: a histomorphometric study in rabbit calvaria.
Allan Zimmermann,André Antonio Pelegrine,Peruzzo D,Martinez Ef,de Mello e Oliveira R,Antonio Carlos Aloise,Lydia Masako Ferreira +6 more
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Phenotype and multipotency of rabbit ( Oryctolagus cuniculus ) amniotic stem cells
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