Open AccessJournal Article
Quantitative Aspects of Thymidine Uptake into the Acid-soluble Pool of Normal and Polyoma-transformed Hamster Cells
TLDR
The uptake of thymidine into an acid-soluble pool in normal hamster embryo cells and hamster tumor cells transformed by polyoma virus was studied and it was found that tumor cells exhibited a 4-fold higher capacity than normal cells to incorporateThymidine as phosphorylated products into this pool.Abstract:
The uptake of thymidine into an acid-soluble pool in normal hamster embryo cells and hamster tumor cells transformed by polyoma virus was studied It was found that tumor cells exhibited a 4-fold higher capacity than normal cells to incorporate thymidine as phosphorylated products into this pool This was correlated with a 5- to 10-fold higher activity of thymidine kinase in the tumor cell lines The uptake process was strongly inhibited by the thymidine analog iododeoxyuridine, partially inhibited by deoxyuridine, and not inhibited by uridineread more
Citations
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Journal ArticleDOI
Transport of nucleosides, nucleic acid bases, choline and glucose by animal cells in culture.
Book ChapterDOI
Permeation of Nucleosides, Nucleic Acid Bases, and Nucleotides in Animal Cells
TL;DR: The chapter discusses the importance of the mechanism of permeation of nucleosides, nucleobases, and nucleotides through the cell membranes of eukaryotes and discusses the carrier model for facilitated diffusion and tests for its applicability to nucleoside and base transport.
Book ChapterDOI
Membrane transport of purine and pyrimidine bases and nucleosides in animal cells.
TL;DR: The chapter discusses the some general principles about transport processes in animal cells, and examines the evidence for specific transport systems for bases and nucleosides, attempting to differentiate between membrane and metabolic events.
Journal ArticleDOI
Thymidine transport by cultured novikoff hepatoma cells and uptake by simple diffusion and relationship to incorporation into deoxyribonucleic acid
Peter G.W. Plagemann,John Erbe +1 more
TL;DR: Results from pulse-chase experiments indicate that a rapid turnover of dTTP to thymidine may be involved in limiting the level of thymine nucleotides in the cell, and indicates that uridine andThymidine are transported by different systems.
Book ChapterDOI
Analysis of nucleotide pools in animal cells.
TL;DR: This chapter discusses the analysis of nucleotide pools in animal cells and presents a model for nucleotidepools, a four-factor model which applies to the thymine nucleotide pool.
References
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Journal ArticleDOI
Distinct mediating systems for the transport of neutral amino acids by the ehrlich cell
Journal ArticleDOI
Phosphate bound to histidine in a protein as an intermediate in a novel phospho-transferase system.
Journal ArticleDOI
Studies on membrane transport, with special reference to parasite-host integration.
TL;DR: In the work to be presented, the authors have examined functional capacities of a cestode parasite, Hymenolepis diminufa, with regard to acquisition of amino acids from a surrounding fluid.
Journal ArticleDOI
Properties of deoxythymidine kinase partially purified from animal tumors.
Edward Bresnick,U.B. Thompson +1 more
TL;DR: The properties of deoxythymidine kinase isolated from tumors of mammalian origin are investigated to supply a molecular basis for the understanding of any regulatory mechanism and perhaps suggest a more important role for this enzyme in the control of deoxyribonucleic acid metabolism.
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