Reactivity of Langerhans cells with hybridoma antibody
Ellen Fithian,Patrick C. Kung,Gideon Goldstein,Marian Rubenfeld,Cecilia M. Fenoglio,Richard L. Edelson +5 more
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This monoclonal antibody was unreactive with non-Ia-bearing epidermal cells and with peripheral blood B cells, T cells, and monocytes but did not bind to 70% of intrathymic lymphocytes, which further distinguish Langerhans cells from classical monocytes.Abstract:
Reactivity of a monoclonal antibody with human Langerhans cells was demonstrated by a double-labeling immunofluorescence technique. Ia-bearing cells of the epidermis (Langerhans cells) were reactive with this antibody both in frozen sections and in cell suspensions prepared from human epidermis. This monoclonal antibody was unreactive with non-Ia-bearing epidermal cells and with peripheral blood B cells, T cells, and monocytes but did not bind to 70% of intrathymic lymphocytes. These observations further distinguish Langerhans cells from classical monocytes. Furthermore, this monoclonal antibody is a highly specific marker for the in vivo identification and in vitro isolation of Langerhans cells.read more
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The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting.
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Lineage promiscuity in hemopoietic differentiation and leukemia.
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Recognition of cluster of differentiation 1 antigens by human CD4 − CD8 >− cytolytic T lymphocyte
Steven A. Porcelli,Steven A. Porcelli,Michael B. Brenner,Michael B. Brenner,Julia L. Greenstein,Cox Terhorst,Steven P. Balk,Steven P. Balk,Paul A. Bleicher +8 more
TL;DR: The results suggest that for a subset of T cells, GDI molecules serve a function analogous to that of MHG class I and II molecules.
References
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Discrete stages of human intrathymic differentiation: Analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage
TL;DR: It is suggested that is it now possible to define stages of T-cell differentiation that can be related to T- cell malignancies in humans.
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Monoclonal antibodies defining distinctive human T cell surface antigens
TL;DR: Three novel nonoclonal antibodies (designed OKT1, OKT3, and OKT4) were generated against surface determinants of human peripheral T cells but differed in their reactivities with T cel- lines.
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Separation of functional subsets of human T cells by a monoclonal antibody.
TL;DR: OKT4 could be a valuable reagent for determining alterations of these functional subsets in human diseases and suggest that the OKT4+ subset represents a helper population and that the OkT4- subset contains the cytotoxic effector population.
Journal Article
Epidermal Langerhans cell density determines whether contact hypersensitivity or unresponsiveness follows skin painting with DNFB.
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Epidermal Langerhans cells are derived from cells originating in bone marrow.
TL;DR: It is demonstrated that, after 3 weeks, most of the Langerhans cells in parental skin which had been transplanted on to F1 hybrids were of recipient origin whereas keratinocytes remained of donor origin; this indicates that the LC are derived from a mobile pool of cells.