Journal ArticleDOI
Recent progress in DNA analysis by capillary electrophoresis.
TLDR
Capillary electrophoresis was found to be instrumental in measuring fine physicochemical parameters pertaining to DNA polyelectrolytes, such as their free mobility and their translational diffusion coefficients.Abstract:
A number of recent developments in DNA analysis by capillary electrophoresis are here reviewed. They include capillary arrays for fast, parallel DNA sequencing as well as microfabricated capillary arrays. Microfluidic chips for DNA sizing and quantitation are also covered, as well as microdevices containing arrays of regular obstacles acting as size-separators during DNA migration. Screening of DNA point mutations by two much improved techniques is also reported: in one case, such mutations are detected (but only on relative short, ca. 60-70 base-long fragments) by free electrophoresis in rather acidic (pH ca. 3) buffers; in the case of single-strand chain polymorphism, an improved technique is described based on near-neutral pH buffers with mixtures of Tris/MES cations/zwitterions. When studying the behavior of inorganic and organic cations in the Debye-Huckel layer of DNA, it was found that the latter (especially a large number of Good's buffers and other zwitterions, such as His) would bind to the DNA filament not only via charge interaction, but also via additional bonds, notably hydrogen bonds, thus altering the electrophoretic (and possibly the biological) behavior of DNA molecules. However, whether or not borate ions would bind to DNA remains still an unsettled question. Finally, capillary electrophoresis was found to be instrumental in measuring fine physicochemical parameters pertaining to DNA polyelectrolytes, such as their free mobility and their translational diffusion coefficients.read more
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Molecular tools--advances, opportunities and prospects.
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DNA-binding of nickel(II), copper(II) and zinc(II) complexes: structure-affinity relationships
Giampaolo Barone,Alessio Terenzi,Antonino Lauria,Anna Maria Almerico,José M. Leal,Natalia Busto,Begoña García +6 more
TL;DR: In this article, a review of the most recent examples of the three metal ions complexed with monodentate and chelating bidentate to tetradentate ligands to DNA is presented, and an attempt has also been made to rationalize the observed trend in the values of the intrinsic DNA-binding constant in terms of structural and chemical features.
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Determination of peptides and proteins in human urine with capillary electrophoresis–mass spectrometry, a suitable tool for the establishment of new diagnostic markers
Stefan Wittke,Danilo Fliser,Marion Haubitz,Sebastian Bartel,Ronald Krebs,Frank Hausadel,Meike Hillmann,Igor Golovko,Peer Koester,Hermann Haller,Thorsten Kaiser,Harald Mischak,Eva M. Weissinger +12 more
TL;DR: Applying CE-MS to the analysis of urine of patients with kidney disease revealed differences in polypeptide pattern, indicating that CE- MS can be applied as powerful tool in clinical diagnostics.
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