Journal ArticleDOI
Recombinant bone morphogenetic protein-2 enhances bone healing, guided by osteopromotive e-PTFE membranes: an experimental study in rats.
Anders Linde,E. Hedner +1 more
TLDR
It was concluded that rhBMP-2 has a strong osteoinductive potential and, in contrast to what was found earlier with other types of BMP preparations, this potential was retained when combining the rhB MP-2 with the osteopromotive membrane technique, yielding better bone healing than with the membrane alone, and at the same time maintaining the bone contour.Abstract:
It has been shown earlier that it is possible to improve bone healing, to regenerate previously existing bone, and to create new bone by means of an osteopromotive membrane technique. The present study addresses the question of whether it is possible to combine this technique with a locally applied factor, stimulatory to osteogenesis. Circular transosseous ‘critical size’ defects in mandibles of rats were either implanted with recombinant human bone morphogenetic protein type 2 (rhBMP-2) or were left empty; half the number of implanted and half the number of empty defects were covered with an expanded polytetrafluoroethylene (e-PTFE) membrane (GORE-TEX®). Results were evaluated after 12 and 24 days of healing by a histomorphological scoring system. Implantation of rhBMP-2 alone resulted in bony bridging of the defect after only 12 days, but also in voluminous amounts of new bone outside the original defect area. When rhBMP-2 was combined with membrane, newly formed woven bone bridged the defect and the bone contour was maintained by the membrane. The combined treatment with membrane and rhBMP-2 demonstrated a significantly better bone healing than with e-PTFE membrane alone at both 12 days and 24 days of healing. It was concluded that rhBMP-2 has a strong osteoinductive potential and, in contrast to what was found earlier with other types of BMP preparations, this potential was retained when combining the rhBMP-2 with the osteopromotive membrane technique, yielding better bone healing than with the membrane alone, and at the same time maintaining the bone contour. This combination may have important therapeutic applications for osseous healing and in reconstructive surgery. The study also shows the importance of an appropriate carrier material when applying stimulatory substances to enhance bone formation in combination with a membrane.read more
Citations
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Journal ArticleDOI
Biomaterial developments for bone tissue engineering
TL;DR: The clinical need for bone tissue-engineered alternatives to the present materials used in bone grafting techniques is presented, a status report on clinically availableBone tissue-engineering devices, and recent advances in biomaterials research are presented.
Journal ArticleDOI
Bone morphogenetic proteins in human bone regeneration.
E.H.J. Groeneveld,E.H. Burger +1 more
TL;DR: Animal studies and laboratory experiments reveal a number of conditions that influence the osteoinductivity of BMP, such as BMP concentration, carrier properties and influence of local and systemic growth factors and hormones.
Journal ArticleDOI
Bone reconstruction: from bioceramics to tissue engineering.
TL;DR: The synthesis of a new generation of biomaterials that can specifically serve as tissue engineering scaffolds for drug and cell delivery is needed and nanotechnology can provide an alternative way of processing porous bioceramics with high mechanical strength and enhanced bioactivity and resorbability.
Journal ArticleDOI
Bone augmentation by means of barrier membranes.
TL;DR: The aim of the present chapter was to review the techniques and membrane materials applied for GBR in conjunction with implant based oral rehabilitation.
Journal ArticleDOI
The role of barrier membranes for guided bone regeneration and restoration of large bone defects: current experimental and clinical evidence
TL;DR: Before clinical applications can be recommended, future research should aim to establish the 'ideal' barrier membrane and delineate the need for additional bone grafting materials aiming to 'mimic' or even accelerate the normal process of bone formation.
References
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TL;DR: Differentiation of the osteoprogenitor cell is elicited by local alterations in cell metabolic cycles that are as yet uncharacterized and may transfer collagenolytic activity to the substrate to cause dissolution of the matrix.
Journal ArticleDOI
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TL;DR: Human complementary DNA clones corresponding to three polypeptides present in this BMP preparation have been isolated, and expression of the recombinant human proteins have been obtained, and each appears to be independently capable of inducing the formation of cartilage in vivo.
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PatentDOI
Bone morphogenetic protein
TL;DR: Bone morphogenetic protein (BMP) made by the process comprising the steps of demineralizing bone tissue; treating the demined bone tissue under aqueous conditions with a water soluble neutral salt and a solubilizing agent for the BMP, the agent being selected from the group consisting of urea and guanidine, and thereby transforming the bone collagen to gelatin and extracting BMP into the solution of solubiliizing agent as mentioned in this paper.
Journal ArticleDOI
The critical size defect as an experimental model for craniomandibulofacial nonunions.
TL;DR: A rationale was postulated for testing bone repair materials (BRMs) using CSDs in a hierarchy of animal models, and it is suggested that testing should be initiated in the calvaria of the rat and rabbit, followed by testing in the mandibles of dogs and monkeys.